FEBRUARY 15-16 - MINUTES OF MEETING 
25 
phage vector." This change had been previously recommended by the RAC 
at its October 1978 meeting. EXiring the 30-day period for public 
response, no comments were received. 
In the publication of proposed changes in the Federal Register on January 
15, 1979, it was noted that the greater flexibility afforded with non-EK2 
lambdoid phage vectors does not in any way compromise safety or contain- 
ment in the final host. The continued requirement for EK2 plasmid vectors 
insures that the final cloning back into the host of origin can be done 
with a minimal introduction of E. coli genetic information. 
Tt was emphasized that the subject of these sections is essentially the 
self-cloning of DNA with the introduction of either lambda or E. coli 
plasmid DNA. For the case of plasmid vectors there might be the possi- 
bility of the vector carrying some E. coli chromosomal DNA. This situation 
is less likely for the lambda bacteriophage vectors. 
The RAC voted 19 to 0 with 2 abstentions to amend Sections III-B-2 and 
III-C-5 of the Guidelines to read as follows: 
"Section III-B-2. Return of DNA segment to Non-HVl host of 
origin * * * 
* * * For a prokaryote which does not exchange genetic informa- 
tion with E. coli (Host B), the following type of experiment 
may be carried out without Host B having been approved as a 
HV1 host: DNA from Host B may be inserted into a lambdoid 
phage vector or into a vector from a certified EK2 host-vector 
system and propagated in E. coli K-12 under the appropriate 
containment conditions [see Section III-A-l-b-(2) ] . Sub- 
sequently, this recombinant DNA may be returned to Host B 
and propagated in Host B under PI conditions. [43] 
III-C-5. Fungal or Similar Lower Eukaryotic Host-Vector 
Systems * * * 
* * * In addition to the experiments described above, the 
following experiments may be carried out without the eukaryotic 
host (Host C) having been approved as an HV1 host: DNA from 
Host C may be inserted into a lambdoid phage vector or into a 
vector from a certified EK2 host-vector system and propagated 
in E. coli K-12 under the appropriate containment conditions 
[see Section III-A-l-(a)-(5)] . Subsequently, this recom- 
binant DNA may be returned to Host C and propagated there 
under PI conditions. [43] * * *" 
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