FEBRUARY 15-16 - MINUTES OF MEETING 
26 
XIX. REQUEST FOR APPROVAL OF SPECIFIC PROKARYOTIC HOST-VECTOR SYSTEMS . 
A. REQUEST FOR CLONING IN BACILLUS SUBTILIS . 
The RAC reviewed a request from Dr. Stanley Cohen of Stanford 
University to permit the cloning in Bacillus subtilis , under 
P2 conditions, of DNA derived from E. coli K-12 and Strepto- 
myces coelicolor using NIH approved Staphylococcus aureus 
plasmids as vectors. No comments were received during the 
30-day period for comment. 
The RAC aqreed that this request had been taken care of by 
the recommendation for certification of a B. subtilis HV1 
system. Therefore, the RAC did not act on this request. 
B. REQUEST FOR CLONING IN STREPTOMYCES COELICOLOR . 
The RAC reviewed a request by Dr. Stanley Cohen of Stanford 
University to permit the cloning in Streptomyces coelicolor , 
under P2 conditions, of DNA from Bacillus subtilis , E. coli 
K-12, and from Staphylococcous aureus plasmid vectors which 
have been approved for use in Bacillus subtilis , using as a 
vector any plasmid indigenous to Streptomyces coelicolor 
or able to replicate in that host by natural biological 
mechanisms. No comments were received during the 30-day 
period for ccmment. 
The RAC noted that it had already approved such experiments 
under P3 conditions under the recommendation passed in item 
VII— D of these Minutes. The RAC voted 17 to 0 with 2 
abstentions to reject the request. 
C. SPECIFIC BACILLUS SUBTILIS EXPERIMENT. 
The RAC considered a request to perm.it cloning in B. subtilis , 
under P2 conditions, of DNA derived from either E. coli K-12 or 
Saccharomyces cerevisiae using NIH-approved EK2 plasmid vectors. 
No comments were received during the 30-day period for comment. 
The RAC aareed that propagation in B. subtilis of E. coli K-12 
DNA on an E. coli vector would be an exempt experiment under 
Section I-E-3. The RAC passed a motion by a vote of 11 to 0 
with 2 abstentions to permit the cloning in B. subtilis , under 
P2 conditions, of DNA derived from Saccharomyces cerevisiae 
using EK2 plasmid vectors provided that an HV1 B. subtilis 
host is used. 
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