FEBRUARY lb-16 - MINUTES OF MEETING 
29 
of containment. Experiments which require the use of whole 
animals will be evaluated by NIH on a case-by-case basis [45]." 
Section III-C-2-b is being added to permit the use of nonviral 
vectors in invertebrate host-vector systems. The revised 
Guidelines permit the use of nonviral vectors for plant host- 
vector systems, but do not address their use for inverte- 
brate systems. No comments were received during the 30-day 
period for comment. The RAC voted 12 to 0 with 2 abstentions 
to recommend the proposed amendment of Section III-C-2. 
XXII. ADDITION OF SECTION III-C-1-f , VERTEBRATE HOST-VECTOR SYSTEMS- 
NON- VIRAL VECTORS . 
Similar to the proposal in XXI above, the RAC recommended that a comparable 
section be added to Section III-C-1 on vertebrate host-vector systems. 
The new sub-section would read as follows: 
"III-C-1-f . Nonviral vectors . Organelle, plasmid and 
chromosomal DNAs may be used as vectors. ENA recombinants 
formed between such vectors and host DNA, when propagated 
only in that host (or a closely related strain of the same 
species), are exempt from these Guidelines (see Section I-E). 
DNA recombinants formed between such vectors and nonviral 
DNA from cells other than the host species require only PI 
physical containment for cells in culture since vertebrate 
cells in tissue culture inherently exhibit a very high level 
of containment. Recombinants involving viral DNA or exper- 
iments which require the use of whole animals will be 
evaluated by NIH on a case-by-case basis [45]." 
No comments were received during the 30-day period for comment. The RAC 
voted 10 to 0 with 4 abstentions to accept this proposal. 
XXIII. DEVELOPMENT OF RETROVIRUSES AS A TRANSDUCING SYSTEM FOR 
MAMMALIAN CELLS . 
A proposal (612) of Dr. David Baltimore, Massachusetts Institute of 
Technology, was submitted to the RAC to assist ORDA in reviewing a case- 
by-case action under the revised 1978 NIH Guidelines. 
This two-part proposal involves the use of a defective Moloney leukemia 
viral genome as a eukaryotic vector under P2 containment conditions. In 
the first part, the thymidine kinase gene of herpes simplex virus would 
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