FEBRUARY 15-16 - MINUTES OF MEETING 
30 
be inserted in the vector. The vector would be very similar to a deleted 
polycna virus, would be restricted in host range, and would require a 
helper virus tor reel icat ion. The second part of the proposal involves 
the construction of recombinants employing the deleted retrovirus DNA 
and DNA segments from either E. coli , yeast, higher cells, or other non- 
viral sources. 
Dr. Baltimore's proposal was reviewed by 3 outside consultants. All 
recommended arproval at the P2 level of containment. Dr. Gottesman 
expressed sore concern as to whether the recombinant could recombine 
with something else and yield something unexpected, such as an active 
virus. 
Dr. Rowe ™ c to approve the use of deleted Moloney leukemia virus 
under r -2 renditions to clone the herpes simplex virus TK gene and 
other non-virai DNA from E. coli , S. cerevisiae , and higher eukaryotes 
provided the helper virus is a murine ecotropic virus. 
Ms. King and Dr. Walters expressed concern that case-by-case decisions 
for virus experiments do not require an opportunity for public comment. 
Dr. Gottesman noted that the Guidelines provide for much simpler pro- 
cedures for these decisions. 
Dr. Rowe aareed to break his motion down into two parts and to allow 
the RAC to vote on them separately. The motion to permit the propagation 
in mouse tissue culture cells of deleted Moloney leukemia viral DNA with 
a herpes thymidine kinase gene insert using an ecotropic helper virus, 
under P2 containment, was passed by a vote of 12 to 0. Ms. Cason and 
Ms. King abstained. 
Dr. Gottesman moved to amend the second part of Dr. Rowe's motion to 
restrict it to the cloning of mouse DNA with the same stipulations as 
for the cloninq of the herpes thymidine kinase DNA. The RAC voted 8 to 
1 with 4 abstentions to accept the proposed amendment. 
The RAC then voted 9 to 1 with 3 abstentions to restrict the second part 
of the proposal only to recombinants involving deleted Moloney leukemia 
viral DNA wit u a mouse DNA insert propagated in mouse tissue culture 
cells using an tcc tropic helper-virus at P2 containment. Dr. Ahmec, 
voted against the motion. 
XXIV. AGROBACTERIUM TUMEFACIENS HOST-VECTOR SYSTEMS . 
A. REQUEST FROM DR. EUGENE NESTER. 
The RAC reviewed a request (618), dated November 28, 1973, 
from Dr. Eugene Nester of the University of Washington for 
clarif leaf ion of containment levels required for recombinant 
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