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Federal Register / Vol. 44, No. 73 / Friday, April 13, 1979 / Notices 
flanking sequences of up to 30,000 base 
pairs in total are eligible for reduction 
by IBC. Larger sequences shall be 
referred to ORDA for approval. 
(d) Recombinant c Jones. IBC's may 
approve requests to recombine two or 
more characterized sequences from any 
source if the sequences have already 
been approved for reduced containment. 
Containment shall be the same level as 
for the characterized clone component 
with the highest containment. 
10. Proposed Exemption for 
Pseudomonas putida and Pseudomonas 
aeruginosa under Section I-E-4 
Dr. N. Omston of Yale University has 
proposed, in accord with Section I-E-4 
of the Guidelines, that Pseudomonas 
putida and Pseudomonas aeruginosa be 
added to the exempt list in Appendix A 
of gram-negative organisms that 
exchange DNA by blown physiological 
processes. Further information 
documenting the exchange of genetic 
information between these two species 
and those in Appendix A is available 
from the Office of Recombinant DNA 
Activities. 
11. Containment Levels for Experiments 
Involving Actinomycetes and Exemption 
for Streptomyces Species that Exchange 
< Genetic Information 
The RAC Working Group on 
Prokaryotic Host-Vectors other than E. 
coli has proposed the following actions 
(reports are available from the Office of 
Recombinant DNA Activities): 
(a) P2 physical containment shall be 
used for DNA recombinants produced 
between members of the Actinomycetes 
group except for those species which are 
known to be pathogenic for man. 
animals or plants. (2A) 
Members of this group of 
microorganisms include the 
Streptomyces and Micromonospora 
genera which produce many medically 
important and beneficial antibiotics. The 
Streptomyces are primarily soil 
organisms and none have been reported 
to be pathogenic in humans. 
(b) Streptomyces species that have 
been shown to exchange chromosomal 
DNA are proposed to be included under 
the exemption category of Section I— E^— 4 
of the 1978 Guidelines. Any recombinant 
DNA molecules that are composed 
entirely of DNA segments from one or 
more of the organisms listed below and 
to be propagated in any of the organisms 
listed below are exempt from the 
Guidelines. (This list is to be separate 
from the other lists of exempt organisms 
in Appendix A.) 
Streptomyces aureofaciens 
Streptomyces rimosus 
Streptomyces coelicolor 
Streptomyces griseus 
Streptomyces cyaneus 
Streptomyces venezuelae 
12. Cloning in Bacillus subtilis and 
Streptomyces coelicolor. 
Dr. Stanley Cohen of Stanford 
University has proposed the following 
actions: 
(a) Bacillus subtilis strains that do 
not carry an asporogenic mutation can 
be used as hosts specifically for the 
cloning of DNA derived from E. coli K- 
12 and Streptomyces coelicolor using 
NIH-approved Staphylococcus aureus 
plasmids as vectors under P2 conditions. 
(b) Streptomyces coelicolor can be 
used as a host for the cloning of DNA 
derived from B. subtilis. E. coli K-12, or 
from S. aureus vectors that have been 
approved for use in B. subtilis under P2 
conditions. 
Dated: April 8. 1979. 
DocuJd S. Fndhckaoo. 
Director. Notional Institute* of Health 
fFF Doc 79-11382 Filed 4-12-7* *45 am) 
BILLING COO€ 4 11 0-04- M 
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