May 21-23 - MINUTES OF MEETING 
5 
Strep tcnry ces griseus 
Streptomyces cyaneus 
Strep torryces venezuelae . " 
During the 30-day comment period no comments were received. The RAC 
considered the criteria for genetic exchange that were set forth as a 
basis for placing a proposed list of Strep tarry ces species in the exemp- 
tion category of Section I-E-4 of the Guidelines. Dr. Campbell explained 
the biology of heterokaryosis. Dr. Novick stated that heterokaryosis, 
except that produced by protoplast fusion, satisfies criterion 2 in 
Appendix A of the NIH Director's Decision Document published in the 
Federal Register on December 22, 1978. Dr. Davies noted that a 
heterokaryon can be maintained with the two nuclei, or the nuclei can 
segregate without recombination to yield the parents, or the nuclei can 
segregate with recombination. He pointed out that recombination is 
common in the case of bacteria; in fact, there are no known cases in 
which recombination has not been observed for Streptomyces species. 
A motion that physiological heterokaryosis between intact organisms 
shall be taken as evidence of genetic exchange under criterion 2 in the 
discussion of Appendix A of the NIH Director's December 22, 1978 Decision 
Document was passed 18 to 0, with 1 abstention. 
Dr. Gottesman made a motion to divide the list of the six proposed 
Streptomyces species into two sublists of three each because the evidence 
for pair-wise exchange was not. as strong between the two sublists as 
the exchange within each sublist. The sublists are as follows: 
Sublist 1 Sublist 2 
Streptomyces aureofaciens 
Strep tony ces rimosus 
Streptomyces coelicolor 
Streptomyces griseus 
Streptomyces cyaneus 
Streptomyces venezuelae 
Any recombinant DNA molecules that are composed entirely of DNA segments 
from one or more of the organisms within each sublist and to be propagated 
in any of the organisms included in that sublist are exempt from the 
Guidelines. (This list is to be separate from the other lists of exempt 
organisms in Appendix A.) 
The motion was passed by a vote of 14 for, none opposed, and 5 abstentions. 
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