May 21-23 - MINUTES OF MEETING 
13 
carrying pBR322. Dr. Novick said that recovery of 
X1776 and xl776 (pBR322) were monitored by different 
techniques, and that an artifact could have been introduced. 
He suggested that discussion on this point be deferred 
until additional data are received. Dr. Campbell said 
that the study involved one group of four volunteers, 
and that there were several variables. Dr. Novick 
noted that perpetuation of the cloned fragment is the 
subject of concern, and that survival is not the same 
as propagating a cloned fragment. He said that when 
survival data are coupled with transfer data, the figure 
is well belcw 10”®. 
X. MODIFICATIONS OF HOST-VECTOR SYSTEMS 
A. Modifications of EK2 Phage Systems 
Dr. Campbell discussed the proposal (684) of the Phage 
Subcommittee on minor modifications of EK2 phage systems. 
He noted that the situation with lambda is complicated 
because more of the safety features are built into the 
vector itself. The RAC voted 16 to 0, with 2 abstentions, 
to approve the modified report that appears as Attachment II. 
It was agreed that the report represents advice to the 
Director, NIH. 
B. Modif ications of EK2 Plasmid Systems 
The RAC discussed the proposal (652) of the Plasmid Sub- 
committee on minor modifications of EK2 plasmid systems. 
Several modifications were made in the proposal. The 
RAC voted 16 to 1, with 3 abstentions, to approve the 
report that appears as Attachment III. 
C. Minor Modifications of Saccharomyces cerevisiae HV2 
Systems 
The RAC reviewed a report (675) on minor modifications of 
Saccharomyces cerevisiae HV2 systems prepared by the 
Working Group on Lower Eukaryote Host-Vector Systems. 
The RAC agreed to several modifications of the proposal 
and voted 14 to 0, with 5 abstentions, to approve the 
report that appears as Attachment TV. 
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