May 21-23 MINUTES OF MEETING 
18 
These comments were discussed at the meeting, and concern v/as expressed 
over the broad nature of the proposal. The use of recombinant DNA methods 
for studying diseases using whole animals or plants was generally supported 
by the RAC. It was the sense of the RAC that this proposal should not 
be extended to human experimentation at this time. 
It was agreed that this revision should appear as a new section of the 
Guidelines, III-C-7. Dr. Kutter proposed that the proposal should be 
divided into two parts, one dealing with higher animals, and one dealing 
with higher plants. The following more restrictive action was proposed 
by the RAC to limit the experiments to easily contained whole organisms 
and to only small portions of viruses: 
" III-C-7. Transfer of Cloned DNA Segments to 
Eukaryotic Organisms . 
III-C-7- a. Transfer to Non-human Vertebrates . DNA 
frcm any nonprohibited source [Section I-D] , except 
for greater than one quarter of a eukaryotic viral 
genome, which has been cloned and propagated in 
_E. coli under appropriate physical containment 
conditions, may be transferred with the E. coli 
vector used for cloning to any eukaryotic cells 
in culture or to any non-human vertebrate organ i an 
and propagated under conditions of physical con- 
tainment comparable to PI and appropriate to the 
organism under study [2A] . Transfers to any other 
host will be considered by the RAC on a case-by-case 
basis [45] . 
III-C-7-b. Transfer to Higher Plants . DNA from any 
nonprohibited source [Section I-D] which has been 
cloned and propagated in E. coli under appropriate 
containment conditions, may be transferred with the 
E. coli vector used for cloning to any higher plant 
organisms (Angios perms and Gyrmosperms) and propagated 
under conditions of physical containment comparable 
to PI and appropriate to the organism under study [2 A] . 
Intact plants or propagative plant parts may be grown 
under PI conditions described under Section III-C-3. 
Containment must be modified to ensure that the spread 
of pollen, seed or other prcpagules is prevented. This 
can be accomplished by conversion to negative pressure 
in the growth cabinet or greenhouse or by physical entrap- 
ment by 'bagging' of reproductive structures. Transfers 
to any other plant organists will be considered on a 
case-by-case basis [45]." 
[ 113 ] 
