May 21-23 - MINUTES OF MEETING 25 
systems may be used under P2 containment 
conditions for shotgun experiments with 
phages, plasmids, and DNA from nonpathogenic 
prokaryotes which do not produce polypeptide 
toxins [34] . For other classes of recanbinant 
DNA experiments with these HV1 systems, except 
for the cloning of complete genomes of eukaryote 
viruses, the S. cerevisiae and N. crassa HV1 
systems and S. cerevisiae HV2 systems may be 
used at the physical containment levels appli- 
cable to EK1 and EK2 systems, respectively.* 
While the RAC generally approved equivalence between these HV systans 
and the EK designations in the Guidelines, there was concern raised 
about the appropriate levels of containment when complete genomes of 
eukaryotic viruses are cloned into these organisms. 
Prior to the RAC considering this issue at its February 1979 meeting, it 
was mistakenly printed in the Federal Register of January 15, 1979 as 
follows : 
"Experiments involving complete gencmes of 
class 1 eukaryote viruses will require 
P3 + HV2 containment levels. Other 
eukaryote viruses are to be handled on 
a case-by-case basis [45]." 
The correct wording should have been, "will require P3 + HV1 or P2 + HV2 
containment levels." 
The RAC in its del iberat ions at the February 1979 meeting took a less 
restricted position and recommended that the following wording be sub- 
stituted for the previous proposal: 
"Experiments involving complete gencmes 
of eukaryote viruses will require P3 + HV1 
or P2 + HV2 containment levels." 
This recommendation passed by a vote of 17 to 1, with 2 abstentions. Since 
this was a major change in the proposed action as it appeared in the 
Federal Register on January 15, 1979, additional opportunity for public 
comment was deemed appropriate. Accordingly, this proposal was published 
in the Federal Register on April 13, 1979 for an additional 30-day comment 
period prior to its presentation at the May meeting. During the 30-day 
ccmment period no comments were received. 
Based on the containment features of these organisms which were previously 
presented in reports to the RAC and the discussion at the February 1979 
meeting, the RAC voted 17 to 0, with 2 abstentions, to accept this proposal. 
[ 120 ] 
