May 21-23 - MINUTES OF MEETING 
28 
Sane members of the RAC expressed concern that recombinant molecules 
containing one-fourth of a viral genome might possibly generate infectious 
virus. Dr. Rowe moved to accept the proposal amended by deletion of 
the portion that referred to exempting recombinant molecules containing 
less than one-fourth of a eukaryotic viral genome. Dr. Novick proposed 
that the motion be amended to include registration and a requirement for 
Pi containment, but with no requirement for prior approval before the 
initiation of experiments. Mr. Thornton said that he could not support 
registration if there is no hazard. Dr. Ahmed then moved to defer con- 
sideration of the proposal until the next meeting. During this discussion. 
Dr. Lewis noted that NSF requires IBC review of exenpt experiments. 
Dr. Ahmed's motion failed to carry by a vote of 4 in favor, 17 opposed. 
Mr. Thornton moved and then withdrew a motion that would have amended 
Dr. Rove's proposal to include a statement that consideration will be 
given at the next meeting to registration of exenpt experiments. 
The RAC then voted 17 to 3, with 2 abstentions, to accept the proposed as 
amended by Dr. Rowe with a minor modification in the wording to include 
" and maintained " in cells, as follows: 
"Those recombinant DMA molecules that are 
propagated and maintained in cells in 
tissue culture and that are derived 
entirely from non-viral components (that 
is, no component is derived from a 
eukaryotic virus) are exempt from the 
Guidelines." 
Drs. Ahmed and Goldstein wished to be recorded as voting against the 
motion. 
XXVI. REVIEW OF PROTOCOLS FOR REQUIRED COUTAINMEOT LEVELS 
A. Construction of Adenovirus 2-SV40 Hybrid Viruses 
Dr. Robert Tjian, University of California, Berkeley, 
requested ORDA to assess the containment necessary to 
construct an Adenovirus 2-SV40 recombinant virus as 
required by section III-C-l-c-( l)-(b) of the 1978 NIH 
Guidelines (646). 
Dr. Tjian proposes to isolate the DMA fragment frem 
the early region of Simian Virus 40 containing the 
gene for the small tumor (t) antigen and insert it 
into a defective Adeno 2 virus. The resultant hybrid 
virus would be unable to replicate because several of 
the Adeno 2 coat protein genes will have been removed. 
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