May 21-23 - MINUTES OF MEETING 
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The SV4U DNA fragment insert will be highly purified, 
precloned into pBR322, and well characterized since the 
entire viral genome has now been entirely sequenced. 
Recombinants between Adenovirus and SV40 can be 
obtained readily in the laboratory employing tissue 
culture systems. 
This proposal was sent to two reviewers. Both reviewers 
recommended P3 containment for developing the recom- 
binants because of the possibility that a nondefective 
virus may be generated. This stringent containment 
could be lowered after it has been established that only 
a defective hybrid virus is produced. 
The RAC in its deliberations took a less restrictive 
position, and recommended a P2 containment level since 
the primary reviewers felt that only a defective hybrid 
virus would be produced which poses no biological hazard. 
The RAC voted to recommend approval of the proposal at 
the P2 level by a vote of 11 for, 3 apposed, with 5 
abstentions. 
B. C onstruction of Recombinant Molecules Between Herpes 
Simplex Virus Thymidine Kinase Gene and Adenovirus Type 
2 and SV4U DNAs (IMUA-93) 
This proposal (647) submitted by Dr. Heiner Westphal of NIH 
may be divided into two parts as follows: 
1. Formation of DNA recombinant molecules 
between the thymidine kinase segment of 
Herpes Simplex virus, the 3.4 kb Bam frag- 
ment, and Adenovirus ENA fragments frcm 
defined regions of the viral genome. After 
propagation in a certified EK2 vector, the 
recombinant molecules will be used to 
transfect cells in culture. 
2. Ligation of SV40 DNA fragments with Adenovirus 
ENA segments and propagation in a certified 
EK2 vector. Following formation of the recom- 
binant molecules, tissue culture cells will 
be transfected to select morphologically 
transformed cells. 
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