Federal Register / Vol. 44, No. 148 / Tuesday, July 31, 1979 / Notices 
45069 
exchange DNA by known physiological 
processes. Further information 
documenting the exchange of genetic 
information between these two species 
and those in Appendix A is available 
from the Office of Recombinant DNA 
Activities. 
4. Cloning in Bacillus subtilis an 
Streptomyces coelicolor. Dr. Stanley 
Cohen of Stanford University has 
proposed the following actions: 
(a) Bacillus subtilis strains that do not 
carry an asporogenic mutation can be 
used as hosts specifically for the cloning 
of DNA derived from E. coli K-12 and 
Streptomyces coelicolor using NIH- 
approved Staphylococcus aureus 
plasmids as vectors under P2 conditions. 
(b) Streptomyces coelicolor can be 
used as a host for the cloning of DNA 
derived from B. subtilis. E. coli K-12, or 
from S. aureus vectors that have been 
approved for use in B. subtilis under P2 
conditions. 
5. Proposed Amendment of Sections 
II-D-l-a-(l) and III-A-l-b-(l). Dr 
Nickolas Panopoulos of the University 
of California, Berkeley has proposed 
amendments of sections II-D-l-a-(l) 
and III-A-l-b-(l). 
The proposed revised sections are as 
follows (new text appears in italics): 
II-D-l-a. HVl. A host-vector system 
which provides a moderate level of 
containment. Specific systems: 
II- D-l-a-(l). EKl. The host is always 
E. coli K-12 or a derivative thereof, and 
the vectors include non-conjugative 
plasmids (e.g., pSClOl, ColEl, or 
derivatives thereof [21-27] and variants 
of bacteriophage, such as \ [28-33], The 
E. coli K-12 hosts shall not contain 
conjugation-proficient plasmids, 
whether autonomous or integrated, or 
generalized transducing phages except 
as specified under Section III-A-l-b- 
(!)■ 
III- A-l-b. Prokaryotic DNA 
Recombinants. 
Ill— A— 1— b— (1). Prokaryotes That 
Exchange Genetic Information [35] with 
E. coli. Those prokaryotes that exchange 
genetic information with E. coli by 
known physiological processes will be 
exempted from these Guidelines if they 
appear on the “list of exchangers” set 
forth in Appendix A (see Section I-E-4). 
For those not on the list, the 
containment levels are Pi physical 
containment + and EKl host-vector. 
In fact, experiments in this category 
may be performed with any E. coli K-12 
vector (e.g., conjugative plasmids). 
However, for prokaryotes that are 
classified [1] as Class 2, the containment 
levels are P2 + EKl. 
When a non-conjugative vector is 
used, the E. coli K-12 host may contain 
conjugative proficient plasmids, either 
autonomous or integrated, or 
generalized transducing phages. In 
general, for experiments in this 
category, the E. coli K-12 host may 
contain such plasmids or phages 
provided that the physical containment 
level is raised one step. 
Additional major actions will appear 
in a subsequent issue of the Federal 
Register. 
Dated: July 24, 1979. 
Donald S. Fredrickson, 
Director, National Institutes of Health. 
[FR Doc. 79-23550 Filed 7-30-79; 8:45 am] 
BILLING CODE 4110-08-M 
