MINUTES OF MEETING - December 6-7 
15 
Dr. Bems discussed the results reported in Table 2, page 1141, 
of tab 787. Dr. Bems said there is no statistically significant 
difference in the tumorigenicity of the recombinant molecule and 
purified polyoma DMA. No obvious enhancement of tumorigenicity 
occurs when the polyoma genome is ligated into a plasmid or 
phage DMA. The data do not support Dr. Newman's contentions. 
Dr. Baltimore moved that Dr. Newman's request (i.e., that 
"containment for tumor virus gene splicing experiments be raised 
to P4 + EK2" ) be denied. The motion was accepted by a vote of 
thirteen in favor, two opposed, with two abstention. Dr. Goldstein 
requested that he be recorded as voting opposed. 
VII. PROTOCOLS REQUIRING ASSIGNMENT OF CONTAINMENT LEVELS 
A. Request to use parvovirus H-l as a vector 
Dr. Baltimore began the presentation of tabs 769 and 791. 
Dr. Solon Rhode of the Institute for Medical Research at 
Bennington, Vermont, requested permission to insert the 
Herpes Simplex thymidine kinase (TK) gene into parvovirus 
H-l. He said H-l has no known pathogenicity except for 
newborn rodents. Dr. Rhode intends to establish the 
defective virus in TK minus hamster cells with a helper H-l 
virus. Dr. Baltimore said H-l becomes defective when a 
segment is deleted and replaced with Herpes TK gene. He 
said Dr. Rhode believes that if the TK gene functions the 
whole system will became TK plus and virus will be produced 
in the presence of helper. Dr. Rhode would then seek recom- 
binants independent of helper virus with the idea that if a 
manmalian origin of ENA replication is incorporated into the 
viral genome, replication would begin at the mammalian origin 
and the production of virus would not require any trans 
funct ion. Dr. Bems noted that Dr. Rhode never mentions the 
source of the DMA being shotgunned. Dr. Gottesman said that 
this proposal is basically a request for use of H-l virus as 
a vector system for cloning in animal cells. She stated 
that little is known about the host range and the replication 
of this virus. Dr. Berns said that Dr. Rhode requests P2 
physical containment for this experiment. However, Dr. Bems 
said he thought P3 containment was more reasonable. 
Dr. Novick moved that this request be denied, but then 
amended his motion to permit the experiment at P3. 
Dr. Walters said that this discussion was very difficult for 
a lay person to understand. He asked whether the investigator 
might specify the source of the DNA. Dr. Goldstein suggested 
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