UNIVERSITY OF SOUTH CAROLINA 
COLUMBIA, S. C. 29208 
SCHOOL OF MEDICINE 
DEPARTMENT OF MICROBIOLOGY 
AND IMMUNOLOGY 
(803 ) 777-56DB 
11 December 1979 
Dr. Donald Fredrickson 
Director 
National Institutes of Health 
Bethesda, MD 2B205 
Dear Dr. Fredrickson: 
I would like to comment favorably on the proposed revision 
of the recombinant DNA guidelines published in the Federal 
Register, November 30, 1979. Experiments with PI contain- 
ment and an EK1 E_. co 1 i host-vector system are minimal risk 
experiments and are one of the most frequent types of re- 
combinant DNA research. If one allows these experiments to 
be monitored solely by the local IBC, a great amount of time 
and effort will be saved without an increase in risk. There- 
fore, I support this proposal wholeheartedly. Also, since 
_E. co 1 i K12 appears to be adapted solely to laboratory growth, 
I support the expanded use of E_. co 1 i K12 as a host organism. 
Thank you for your continued efforts to update and simplify 
the recombinant DNA guidelines. 
Sincerely , 
Bert Ely 
Associate Professor 
BE : cmp 
[ 450 ] 
The University of South Carolina: USC Aiken; USC Salkehatchie, Allendale; USC Beaufort; USC Columbia; Coastal 
Carolina College, Conway; USC Lancaster; USC Spartanburg; USC Sumter; USC Union; and the Military Campus. 
