AREA CODE 60 
PHONE 262-217 
MEDICAL CENTER 
UNIVERSITY DF WISCONSIN • MADISON, WISCONSIN 537D6 
McARDLE LABORATORY 
FOR CANCER RESEARCH 
December 14, 1979 
Dr. Donald Fredrickson 
Director 
National Institutes of Health 
Bethesda, MD 20205 
Re: Proposed Guidelines for Recombinant DNA Research 
Dear Don: 
I have read the proposed guidelines for research on recombinant DNA 
published in the Federal Register of November 30, 1979. I am glad to see 
this simplification in the regulations governing research with recombinant 
DNA. It is clear from all of the research that has been carried out using 
recombinant DNA technology that the only "surprises" have been to make un- 
planned expression even less likely than thought. It is clear that the 
earlier fears of effects of eukaryotic DNA in E. col i were exaggerated. 
Now the major problem in this work is the administrative one. Our re- 
search was held up more than six months because of waiting for changes in the 
guidelines. We still find great difficulty in receiving responses to "case 
by case" requests. 
I hope that the acceptance of these revised guidelines will make admini- 
stration of the research that does not fall under section III-O simpler. 
One minor point: for people not used to reading the language of legal 
and federal regulations, it is not immediately obvious that section III-O 
overrules later sections and that, therefore, all work using the systems 
described in that section are carried out in PI, EK1 conditions, even if 
mentioned further later in the document. 
With bes't regards 
Sincerely yours 
Howard M. Temin 
HMT/btk 
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