BROOKHAVEN NATIONAL LABORATORY 
hill 
a in 
ASSOCIATED UNIVERSITIES, INC. 
Upton, New York 1 1 973 
Department of Biology 
(516) 345-3390 
December 19, 1979 
Dr. Donald Fredrickson, Director 
National Institutes of Health 
Bethesda, Maryland 20205 
Dear Dr. Fredrickson: 
I strongly support revision of the Guidlines to permit reduced 
containment levels for experiments using the E. coli K-12 
host-vector systems (Federal Register, November 30, 1979). 
I consider the evidence overwhelming that these experiments 
pose no significant hazard. 
The restrictions imposed by the Guidlines have been a substantial 
impediment to realization of the benefits offered by the use of 
these new techniques. While such caution may have been warranted 
at the outset, I feel it can no longer be justified. 
Sincerely yours, 
F. William Studier 
Senior Biophysicist 
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