AR EA CODE 6 
PHONE 262-21 
McARDLE LABORATORY 
FOR CANCER RESEARCH 
UNIVERSITY □ F WISCONSIN • MADISON, WISCONSIN 53706 
December 21, 1979 
Dr. Donald Fredrickson 
D i rector 
NIH 
Bethesda , HD 20205 
Dear Don: 
I read your proposed Guidelines in the Federal Register 44 (1979) 
69210 and I have the following comments and recommendations (and 
corrections) : 
(1) Two paragraphs in I-D-6 should be combined into one paragraph, since 
otherwise the paragraph starting with "Experiments in these categories ..." 
appears to relate only to I-D-6 and not to I-D-1-to-6. 
(2) In title to II-D-2-b: Use lower-case £ in E . co 1 ? . The same correction 
should be made on p. 69236, 1st Column, line 9 up. 
(3) I support the addition of section III-O, even it became unnecessarily 
cumbersome, probably reflecting some political, not scientific, 
compromises (pp. 69218 and 69245). 
(4) The final paragraph of section III-O is helpful since it would generate 
a list of "eschangers" which were not included in Appendix A. NIH should 
instruct the local IBC's to tabulate such "exchangers" and supply this 
information to ORDA for future amending Appendix A. 
(5) In Table IV entry 3: it should be Human Ad2 or Ad5 (not Ad2 + Ad5, 
which makes no sense). The footnote (**) should be explained as in Gene 
5_ (1979) p. 190 footnote f. A- , 
(6) The reference 33A should be updated by including on p. 69230 reference 
to: 
Enquist, L. and W. Szybalski (1978). Coliphage lambda as a safe vector for 
recombinant DNA experiments . In "Viruses and Environment" (L Kurstak 
and K. Maramorosch, eds.) Academic Press, New York, pp. 625 - 652. 
A reprint is enclosed. E , 
(7) Why are organisms classified as Class 3,4, or 5 specified in I-D-1 , 
and V. 2, but there are only classes 3 and 4 (no 51) in Appendix B? This is 
confus i ng . 
[ 580 ] 
