bacterial neomycin resistance (Neo R ) gene cloned Into the Mo-MuLV backbone of 
N2 was derived from Tn5 (Southern and Berg. 1982) by a Bglll and BamHI digest 
with the addition of EcoRI linkers. 
The SAX vector was generated by inserting a fusion gene of a primate (the 
SV40 early gene) promoter and a human ADA cDNA Into the unique Xhol site of N2 
(Kantoff et al.. PNAS. 1986; copy supplied in Appendix B). The SV40 early 
promoter was a 400 base pair (bp) Kpnl - Hindlll fragment placed in the same 
orientation upstream from a 1300 bp full-length ADA cDNA [EcoRI - AccI fragment 
of clone ADA 211 (Adrian et al.. 1984)]. A complete nucleotide sequence 
combined with a detailed restriction enzyme map of SAX has been generated (see 
Appendix A) . 
(2) What regulatory elements does the construct contain (e.g.. 
promoters, enhancers, polyadenylatlon sites, replication origins, 
etc.)? 
Unmodified Mo-MuLV LTRs and the SV40 early gene enhancer/promoter region. 
See I.B.l.a.(l) above. 
(3) Describe the steps used to derive the DNA construct. 
See I.B.l.a(l) above. 
b. What Is the structure of the material that will be administered to the 
patient? 
(1) Describe the preparation, structure and composition of the 
materials that will be given to the patient or used to treat 
the patient's cells. 
(a) If DNA. what Is the purity (both In terms of being a single 
DNA species and In terms of other contaminants)? What tests 
have been used and what Is the sensitivity of the tests? 
Not applicable 
Recombinant DNA Research, Volume 12 
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