Hinfl MspI 
Drain 
Mnll 
Hpall 
Bbvl 
68 
ii i ii 
TCACCAAACCACCCAGTGGGACTCCTCTCTCCCCTACCCAACTTCCACCGGCGGGAACGGAAAATAG 
83 
102 
116 
123 
88 
116 
Fnu4HI Ddel AccI NlalV 
HphI 
Mnll Mnll 
135 
i 1 i i i ii 
GCTGCTAAACTTAGTAGACGGAGCCTACCAAGCCCTCAACCTCACCAGTCCTGACAAAACCCAAGAG 
137 145 150 157 
169 
179 185 
Mael 
SinI 
Sau96I 
NlalV 
NlalV 
Eco47I 
Drall 
Avail 
I I I 
215 
224 
224 
224 
225 
226 
224 
224 
ScrFI 
Rsal 
EcoRII NlalV 
BstNI 
BanI 
Asp718 
Apyl Kpnl 
II III 
202 TGCTGGTTGTGTCTAGTAGCGGGACCCCCCTACTACG AAGGGGTTGCCGTCCTGGGTACCTACTCCA 
254 
261 
257 
257 
254 
252 
254 
259 
259 
Mnll 
286 
Pall Tthllll 
Haelll Mnll Hindi 
I III 
302 
302 
313 318 
322 
Maelll 
269 ACCATACCTCTGCTCCAGCCAACTGCTCCGTGGCCTCCCAACACAAGTTGACCCTGTCCGAAGTGAC 
331 
MspI 
Hpall 
I 
Hinfl 
336 
336 
345 
Sau96I 
Pall 
Haelll 
Drall 
336 CGGACAGGGACTCTGCATAGGAGCAGTTCCCAAAACACATCAGGCCCTATGTAATACCACCCAGACA 
379 
380 
380 
379 
Recombinant DNA Research, Volume 12 
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