PstI 
Pall 
Haelll 
Ball 
I 
Thai 
Pall 
Haelll FokI 
FnuDII NlalV 
Fnu4HI BanI 
III III 
2428 
2434 
2434 
2434 
2456 2465 
2458 2467 
2455 2463 
2455 
2458 
Hph 
I 
2413 ACCTTCTGCTCTGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAACCGAG 
247 
2480 
ScrFI 
Mnll 
Sau3AI 
ScrFI 
Sau96I 
Pall 
Haelll 
Tthllll 
SinI 
ScrFI 
Sau96I 
NlalV 
EcoRII 
Eco47I 
Drall 
EcoRII 
Ndell 
EcoRII 
BstNI 
BstNI 
Mbol 
BstNI 
Avail 
Apyl 
i i 
Cpfl HphI 
Apyl 
Nlalll 
Apyl 
ii i i i i i i i i i i i 
ACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCGCATGGACACCCAGACCAGGTCCC 
2492 
2499 2505 
2517 
2528 
2540 
2492 
2499 
2517 
2542 
2490 
2499 
2515 
2540 
2492 
2499 
2520 
2542 
2492 
2520 
2519 
2517 
2542 
2538 
2544 
2542 
2540 
2542 
2540 
ScrFI 
Maelll 
EcoRII 
BstNI 
Apyl 
Styl 
ScrFI 
EcoRII 
BstNI 
Apyl Mnll 
I I I 
2554 
2560 
2560 
2558 
2560 
2568 
2590 2595 
2590 
2588 
2590 
Rsal Dde 
I I 
2547 CTACATCGTGACCTGGGAAGCCTTGGCTTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACACCCT 
2607 261 
[124] 
Recombinant DNA Research, Volume 12 
