Bbvl 
I 
SfaNI 
Fnu4HI 
I I 
Fnu4HI 
2844 
2858 
2855 
2869 
MspI 
Hpall 
2815 GGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGG 
2879 
2879 
ScrFI 
NlalV 
Neil 
Narl 
MspI 
HinPlI 
Hhal 
Haell 
HinPlI Cfol 
Hhal BanI Hpall 
Cfol Ahall 
II I I I I I I 
2891 2897 
2891 2896 2901 
2889 2899 
2900 
2899 
2897 
2901 
2897 
2901 
2898 
2901 
NlalV 
MspI 
Hpall 
BanI 
2931 
2929 
2929 
2933 
Mnll 
I 
2882 CTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGC 
2947 
PstI 
I 
2950 
Thai 
HinPlI 
Hhal 
FnuDII 
Fnu4HI Pall 
Fnu4HI Haelll 
Cfol Bbvl Ball 
I I I I I I 
2963 
2959 
2964 
2963 
2963 
2961 
2963 
2970 
2979 
2979 
2979 
PvuII 
HinPlI 
Hhal 
FspI 
Fnu4HI 
Cfol 
Alul 
I I I I I 
3003 
3000 
3001 
2999 
3000 
2998 
3003 
TaqI Tth 
HgiAI 
Bbvl 
I I I 
2949 AGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGT 
3012 
3010 
3010 301 
[ 126 ] 
Recombinant DNA Research, Volume 12 
