XhoII 
Sau3AI 
Ndell 
Mbol 
Cpfl 
ScrFI 
Neil 
MspI 
Maelll Bbvl Fnu4HI Hpall Cpfl HphI 
II I II I I 
3016 TGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCT 
3017 3028 3042 3060 3068 3075 
3060 
3061 
3061 
3068 
3068 
3068 
3068 
3068 
Sau3AI 
Fnu4HI Ndell 
Nlalll Fnu4HI Mbol 
SfaNI Bbvl Fnu4HI Cpfl 
III III I 
3083 CACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATC 
3110 3121 3129 3146 
3116 3132 3146 
3135 3146 
3146 
MspI SfaNI Rsal 
Hpall TaqI TaqI HgiAI 
I I I I I I 
3150 CGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGC 
3150 3166 3190 3200 
3150 3196 3203 
Thai 
TaqI 
HinPlI 
Sau3AI 
Sau3AI 
SfaNI Hhal 
MspI 
Ndell 
Ndell 
FnuDII 
Hpall 
Mbol 
Mbol 
MboII Cfol 
FokI 
Cpfl 
Cpfl 
FokI 
Banll 
EcoR 
i i i i i i i i i i i i 
CGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCC 
3221 
3227 
3236 
3246 
3262 
3282 
3217 
3227 
3236 
3258 3266 
3217 
3227 
3236 
3264 
3227 
3236 
3260 3266 
3226 
3264 
3264 
Recombinant DNA Research, Volume 12 
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