3284 
3351 
3418 
3485 
Thai 
HinPlI 
HinPlI 
Hhal 
Hhal SphI 
FnuDII 
XhoII 
Sau3AI 
Styl 
ScrFI 
Cfol 
Ndell 
SfaNI 
BstNI 
Cfol Nlalll 
Mbol 
Ncol 
Apyl 
BssHII Mnll 
Cpfl 
Maelll Nlalll 
i i i t i i i i i i i 
AGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGA 
3284 
3294 3304 
3314 
3323 3333 
3284 
3296 3302 
3314 
3329 
3284 
3298 
3314 
3326 
3296 
3314 
3329 
3296 3302 
3298 
3294 
3296 
3296 
3314 
Nlalll 
Pall 
Haelll 
Fnu4HI 
Hinfl TaqI 
I I 
Pall 
Nael 
MspI 
Hpall 
Haelll 
I I I 
3359 
3371 
3370 
3370 
3382 3388 
3397 
3398 
3398 
3399 
3397 
SinI 
Sau96I 
Eco47I 
Avail 
I 
ATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCG 
3413 
3413 
3413 
3413 
MboII 
Alul Fnu4HI 
I I I 
CTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGC 
3461 
3467 
3468 
HinPlI 
Hhal 
Fnu4HI 
Mnll Fnu4HI Hinfl Cfol 
I I I I I I I I I 
TTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG 
3498 3508 3516 3526 
3522 
3526 
3524 
SfaNI 
Bbvl 
3533 
3535 
MboII 
3548 
[ 128 ] 
Recombinant DNA Research, Volume 12 
