4423 
Pall Nael 
Mnll 
ScrFI 
Neil 
MspI 
Hpall 
HinPlI 
Hhal 
Haell 
Haelll 
Fnu4HI 
I I 
MspI 
Hpall 
Fnu4HI 
Fnu4HI 
I I 
Fnu4HI Mnll 
Cfol Bbvl 
I I I I I I I 
GGGAGCGGCCACCGAGCCGGCAGAGACCCACCGAGCGGCGGCGGAGGGAGCAGCGCCGGGGCGACGA 
4429 
4431 
4431 
4440 
4440 
4441 
4459 
4462 
4459 
4478 4485 
4474 4481 
4479 
4478 
4476 
4479 
4479 
4480 
4480 
Styl 
Sau96I 
NlalV Pall 
Ncol Haelll TaqI Nlalll 
BanI Nlalll Ahall Hgal Mael 
I I I II I II III 
4490 GGGCACCATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCACCTAGACGGA 
4492 4500 4507 4515 4549 
4496 4501 4517 4543 
4494 4501 
4500 
4496 
Xhol 
Sau3 
Ndel 
Mbol 
Cpfl 
BstI 
BamH 
4555 
4555 
4555 
4555 
4555 
4555 
4555 
NlalV 
Mnll Mnll 
Sau3AI 
Ndell 
Mbol 
Cpfl 
4601 
4601 
4601 
Mnll 
Mnll 
Bbvl 
Alul 
I I 
4557 TCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGCCCTCCCAGCTAACACAG 
4557 4585 4590 4601 4615 
4619 
4618 
4619 
Recombinant DNA Research, Volume 12 
[133] 
