5160 
5227 
5294 
5165 
ScrFI 
EcoRII 
BstNI 
Apyl 
Mnll Fnu4HI 
I I II 
GAAGTAGTAAAAGAGGCTGTGGACATACTCAAGACAGAGCGGCTGGGACACGGCTACCACACCCTGG 
5200 
5224 
5224 
5222 
5224 
ScrFI 
Sau96I 
Pall 
Haelll 
EcoRII 
Drall 
BstNI 
Bbvl 
Apyl MboII 
I I I I I I 
Fnu4HI 
Fnu4HI 
XhoII 
TaqI 
Sau3AI 
Ndell 
Mbol 
Cpfl 
Nlalll Bglll 
I 
I I 
SinI 
ScrFI 
Sau96I 
EcoRII 
Eco47I 
BstNI 
Avail 
Apyl 
AAGACCAGGCCCTTTATAACAGGCTGCGGCAGGAAAACATGCACTTCGAGATCTGCCCCTGGTCCAG 
5233 5239 
5237 
5233 
5235 
5231 
5236 
5236 
5235 
5233 
5251 
5254 
5268 
5276 
5276 
5276 
5276 
5276 
5273 
5276 
5286 
5288 
5286 
5288 
5284 
5288 
5286 
5288 
ScrFI 
NlalV Tthllll 
Mnll ScrFI 
EcoRII EcoRII 
BstNI MspI SphI BstNI 
Alul BanI Apyl Hpall Nlalll Apyl 
I I I I I I I II 
CTACCTCACTGGTGCCTGGAAGCCGGACACGGAGCATGCAGTCATTCGGCTCAAAAATGACCAGGTC 
5294 5305 5310 5317 5332 5356 
5310 5317 5332 5356 
5308 5354 
5308 5356 
5307 5356 
5310 
Recombinant DMA Research, Volume 12 
[ 137 ] 
