Sau3AI 
Ndell 
TaqI 
Mbol 
Clal 
Cpf I EcoRI 
Nlalll 
Alul 
Banlll 
EcoRV 
l l i i i i 
AGGATCGGAATTCTCATGTTTGACAGCTTATCATCGATTAGTCCATTTGTTAAAGACAGGATATCAG 
5966 5972 
5982 
5990 
5998 
6026 
5966 
5998 
5966 
5966 
5998 
SinI 
ScrFI 
Sau96I 
EcoRI I 
Eco47I 
BstNI 
Avail 
HphI 
PvuII 
Apyl 
Mael 
Hinfl 
Alul 
Rsal 
i i i i ii i i 
TGGTCCAGGCTCTAGTTTTGACTCAACAATATCACCAGCTGAAGCCTATAGAGTACGAGCCATAGAT 
6037 
6033 
6043 
6051 
6055 
6069 
6069 
6085 
6037 
6033 
6035 
6033 
6037 
6033 
6098 AAAATAAAAGATTTT ATTTAGTCTCCAG AAAAAGGGGGGAATG AAAG ACCCCACCTGTAGGTTTGGC 
6165 
Nhel 
Mael 
Alul 
Alul Maelll 
Nlalll Ddel 
AAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAAATACATAACTGAGAATAGAGAAGTTCA 
6168 6177 6200 6214 
6172 
6169 
6168 
Recombinant DNA Research, Volume 12 
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