Listing of Supplies 
Tissue culture medium: DMEM (Gibco, Cat. No. 320-1965) - see attached sheet 
Fetal Bovine Serum (FBS) - low level endotoxin and hemoglobin, protected 
from photoxic oxidation. 0.1 micron pore size triple filtered. Hyclone 
Laboratories. Logan. Utah 84321. 
Phosphate Buffered Saline (PBS) - Dulbecco's calcium and magnesium free, 
Gibco Laboratories. Grand Island. NY 14072 
Heparin - preservative free, porcine intestinal mucosa, pH 5. 0-7. 5. 
sterile, non-pyrogenic. 1000 USP units/ml in normal saline. Invenex. Melrose 
Park. II 60160. 
D 
Lymphocyte Separation Medium (LSM )- Sodium diatrizoate/Ficoll density 
1.077-1.080 g/ml . Litton Bionetics, Kensington. MD 20795. 
Protamine - Protamine sulfate injection. USP, Eli Lilly and Company. 
Indianapolis, IN 
Other materials: 
Tissue culture plates 
Pipettes 
Polypropylene tubes 
Cellulose acetate filters (e.g.. Corning, 25942) 
Syringes 
Bone marrow aspriation needles 
Intravenous access lines 
These are all standard tissue culture or hospital supply equipment. 
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Recombinant DNA Research, Volume 12 
