DULBECCO'S 1 MODIFIED EAGLE MEDIUM 
150 
□ u Ta 
With Low Glucose 
With High Glucose 
Liquid (IX) 
Powder 
Liquid (IX) 
Powder 
Cat. No. 320-1885 
Cat. No. 430-1600 
Cat. No. 320-1965 
Cat. No. 430-2100 
COMPONENT 
mg/L 
mg/L 
mg/L 
mg/L 
INORGANIC SALTS: 
CaClj (anhyd.) 
200.00 
200.00 
200.00 
200.00 
Fe(NOj), • 9H 3 0 
0.10 
0.10 
0.10 
0.10 
KCI 
400.00 
400.00 
400.00 
400.00 
KNOj 
— 
— 
— 
— 
MgS0« (anhyd.) 
— 
97.67 
— 
97.67 
MgSOa • 7HjO 
200.00 
— 
200.00 
— 
NaCI 
6400.00 
6400.00 
6400.00 
6400.00 
NaHCOj 
3700.00 
— 
3700.00 
— 
NaHjPOa • HjO 
125.00* 
125.00* 
125.00* 
125.00* 
Na 2 SeOj 5HjO 
— 
— 
— 
— 
OTHER COMPONENTS: 
O-Glucose 
1000.00 
1000.00 
4500.00 
4500.00 
Phenol red 
15.00 
1500 
15 00 
15.00 
HEPES 
— 
— 
— 
— 
Sodium pyruvate 
110.00 
110.00 
— 
— 
AMINO ACIOS: 
L-Alanine 
— 
— 
— 
— 
L-Asparagine - HjO 
— 
— 
— 
— 
L-Arginine • HO 
84.00 
84.00 
84.00 
84.00 
L-Aspartic acid 
— 
— 
— 
— 
L-Cynine 
48.00 
— 
48.00 
— 
L-Cystine 2HCI 
— 
62.57 
— 
62.57 
L-Glutamic acid 
— 
— 
— 
— 
L-Glutamine 
584 00 
584.00 
584.00 
584.00 
Glycine 
30 00 
30.00 
30.00 
30.00 
L-Histidine HCI • H,0 
42.00 
4200 
42.00 
42.00 
L- Isoleucine 
105.00 
105.00 
105.00 
105.00 
L- Leucine 
105.00 
105.00 
105.00 
JQJLQQl 
1 .1 yeirsa MP1 
*4600- 
-T46.TJ0 
146.00 
146.00 
L-Methionine 
30.00 
30.00 
30.00 
30.00 
L-Phenylalanine 
66 00 
66.00 
66.00 
66.00 
L-Proline 
— 
— 
— 
— 
L- Serine 
42 00 
42.00 
42.00 
42.00 
L- Threonine 
95.00 
95 00 
95.00 
95.00 
L-Tryptophane 
16.00 
16.00 
16.00 
16.00 
L-Tyrosirte 
72.00 
— 
72.00 
— 
L-Tyrosine (Duodium salt) 
— 
103.79 
— 
103.79 
L-Valine 
94 00 
94.00 
94.00 
94.00 
VITAMINS: 
Biotin 
— 
— 
— 
— 
D-Ca pantothenate 
4.00 
4.00 
4.00 
4.00 
Choline chloride 
4.00 
4 00 
4.00 
4.00 
Folic acid 
4.00 
4 00 
4.00 
4 00 
i-lnositol 
7.20 
7.20 
7.20 
7 20 
Nicotinamide 
4.00 
4.00 
4 00 
4 00 
Pyrtdoxal HCI 
4.00 
4 00 
4 00 
4 00 
Riboflavin 
0 40 
0 40 
0 40 
0 40 
Thiamine HCI 
4.00 
4 00 
4 00 
4 00 
Vitamin Bi] 
— 
— 
— 
— 
'Oulbecco R.. Freeman G.. Virology. 8 396 (19S9) Smith J 0 , Freeman G.. Vogt M and Dulbecco R . Virology. 12 185-196 (I960) Tissue 
Culture Standards Committee. In Vitro, Vol. 6. No 2. P 93 
*Valuet shown are in conformance with the Tutue Culture Standardi Committee. 7n V'tro, 9. No 6 119701. 
Recombinant DNA Research, Volume 12 
[ 165 ] 
