kilobase pairs 
l«c*5 
«t 1*0 I 
ntff r rr * 7Yr“ 
*4 N 
Vo lam 
u 
4 
i'll ft 
■titii it t i t 
n i 
i 1 
I 
LTB 
k k kk«k *c 1*0 a 
pot 
u I m r> 
it* 
s i 1 1 1 * — +- 
00 1.0 24 JO 40 SO 
H 1 ( ir 
tO 74 14 U 
KILOBASE PAIRS 
Analysis of the genomes of eco tropic and amphotropic MuLV’s isolated from the California wild 
mice. Each pair woe isolated from the same mouse. 
Common restriction endonuclease sites iMre indicated with short 
kies and the name of the enzyme indicated above. Enzyme sites that are different within the pair art 
indicated with longer lines. Abbreviations' E, EcoRI', Ps, PstI, Sm, SmaJ, K, Kpnl, PI, Pvul, Ss, SstU, Ba 
BamHI. He, Hindi ; Hp. Hpal; Hd, Hmdlll, Sc, Sadi SI, Sail, Pv, Pvul I ; Xh, Xhol, Cl, Clal, Xb. Xbal; Be, 
BcU. 
Fig. 6 . Amphotroplc viruses were grown In the permissive Mus Carol 1 or Hus 
dun 1 1 cell lines, which were acutely Infected with Mo-MuLV from chronically 
Infected cells. DNA was extracted on day 16 to 18 after Infection, and the 
viral DNA was separated from genomic DNA by velocity sedimentation of denatured 
genomic DNA In the presence of SDS and NaCl , while taking advantage of the 
spontaneous renaturatlon of high copy number, low sequence complexity viral DNA 
which remained In the supernatant (Hlrt. 1967). The viral DNA supernatant was 
preparatlvely el ectrophoresed (Rands et al.. 1981). Purified form I 4070A 
viral DNA was linearized with EcoRI and cloned Into the EcoRI site of pBR322 
(Chattopadhyay et al., 1981). The DNA clones were restricted and analyzed on 
32 
nitrocellulose paper after electrophoresis and transfer, with P cDNA probes. 
Restriction endonuclease maps of p4070A amphotroplc virus are shown 
(Chattopadhyay et al., 1981). 
Recombinant DNA Research, Volume 12 
[1771 
