VaWri o Vi- ^3, W 1 / 
165 
Fig I Construction of a retroviral shuttle vector containing the coding sequence of human ADA. The AD A -coding sequence was 
iaoiaied from the ADA cDNA clone pLL (V'aieno et al., 1 9S-*b) which was inserted into the BamH\ aite of pZIP-NeoSV(X)l (Cepko 
et al.. 1984) This clone was designated pZIP-ADA LTR long teratmal repeat. 3'as 3 aplice acceptor used in the formation of the 
Moloney Murine Leukemia Vmis en mRN'A, aeo: the Tnf aec gene (Davies and Jimenez. 1980, Colbere-Garaptn et aL. 1981, Southern 
and Berg. 1982). 
Gsf Vc zkJi 
cZ g-hec Svfx 
L j 
r- 
j 
i ;; : 
pZiP-StcSV(B)! 
II SS 
1 . Strjcr^-e of me ZFNec Shjrae Vectors 
The cZP crwr-s done of -t“-.a-r e* a (I9e2 *as jsec as me scarce 
of al MJiijLV seojevces Fro m me 5' L~ to me 5a— m s.-e was 'smaiy 
me 0-1 C «£ -agmem ort pZP .me Pst see at raideotoe 56c >'vmo et 
« . 19B - ' was ctxtrened to a Bar- rt s-*e o, me aooton of a The 
3’ SS fragment er.e-. 2 s from me MAAuLV Bgf I see a- ndeotoe 509 to 
me Xba i see a; -o&eotoe S’Sc a no encooes a 3 soce acceptor 
secuence jtAzed r me tormaton of me sjogenomuc erv RNA The nec 
fragment was ongm-alr, the 1 4 tt> Hrxj t-Sa fragment -am. Tn5 (Jorgerv 
se- et a! 1975 Sojme" ane 5e-g '962 Seo-*nces aoe.ee SVor were 
denied from pSvOO '.fetor et a . 1961 anc ereno from Svac -vjcaeoMe 
16C to tufeoue 5171 (Tocze 1981) The oSBor seojtrces ereno from 
rooectoe 252' to nuoeoooe 2'C2 (SdlcWe 1978) (the onenoaon o< these 
seo^e-ces has "o' see- established The 3’ enc of me .actor was taken 
frer-. pZP frer- me -ea sue at nudeotoe 7197 tc me 3' end of the cZP 
pro^rus The reskicfron sues rat created amg me consr-coor are 
as shown For reference o' pZP sees me standard m-kalv map car. be 
consoled (Shmck et a ".961, Wess et a . i962i 
[ 192 ] 
Recombinant DNA Research, Volume 12 
