W'" 
AA/ 
Xbol 
Xbol 
f*-TR 1 lo^R^'wi IlTbI 
ZipDMFR* 
Xbol 
V\A- 
Xbnl Xbol 
* ADA * 
ZlpDHFR*- ADA 
Xbol 
- / W 
Xbol 
-E™1 ^ 
Xbol 
♦ 
SVp» 
3*m 
ADA 
Xbol 
♦ 
Xbol 
Jtih. 
■AA/ 
Zip DHFR* - SVADA 
FlC. 1. Structure of the ZlpDHFR* vector and ADA recombi- 
““ , pMflSii gat, fa ft 
704 Biochemistry: Friedman 
Proc. Mall. Acad. Sci. USA 82 (1985) 
»** 
ftroRI 
4ccl FcoRI 
Mwdlll 
Btm HI 
FcoRI M«tt*yUw 
Add fcoRI B*mH I Adapwn 
•i* T4 ON A L«M 
FcoRI 
Cot pun+r 
Aon Ml 
Accl 
Add FcoRI link on 
»;th T4 DMA I 
FcoRI 
ItoUn 1330 bp t ragman t 
FcoRI 
FcoRI 
Fic. 1. Construction of retrovirus vectors containing human ADA sequences. pZIP-Neo SV(X)1 is described in ref. 23. pADA 211 (11) 
contains the ADA 211 cDNA subcloned in pBR325 (31) Orientations of ADA sequences in pSVX-ADA 1 and 2 were determined by digestion 
with Bgl II. NEO. TnJ neomycin resistance gene: 3'SS. Moloney MuLV 3' splice site; SV, simian virus 40 origin of replication; UT. 
untranslated region; bp. base pairs; pBRori. pBR322 origin of replication. 
Recombinant DNA Research, Volume 12 
[197] 
