l »'l 
t 
T2- 
Effects of Diff er e n t Promoters upcn ADA Gene Expression in Imnortalized ADA T Iynphocytes 
Cells were oocultivated with vector-producing fibroblasts, selected in G418 for three 
w eeks and then assayed for ADA activity. 
T Cell Line 
Vector (Promoter-Gene) 
ADA Activity 1 
% Normal 
Normal 
no vector 
495 
100% 
ADA-deficient 
no vector 
3 
<1% 
ADA-deficient 
N2 (Lm-Neo r gene) 
4 
<1% 
ADA-de f icient 
SAX (SV40 early gene-hADA 2 ) 
1580 
319% 
ADA-deficient 
CAX (Cytanegalovirus inmediate 
early gene-hADA) 
1484 
300% 
ADA-de f icient 
HAX (Murine MHC locus 3 H2D-hADA) 
1694 
342% 
ADA-deficient 
TAX (Thymidine kinase-hADA) 
725 
146% 
ADA-deficient 
BAX (Human beta-actin-hADA) 
943 
193% 
1. nanomoles inosine produced/minute/ 10 8 cells 
2. hADA = human ADA cENA 
3. MHC locus = major histoccmpatibilty locus 
Recombinant DNA Research, Volume 12 
[ 201 ] 
