ADA & NEO** EXPRESSION IN DAY 10 MOUSE SPLEEN FOCI 
VECTOR 
DNA 
DOT BLOT 
ADA 
STARCH GEL 
NPT ASSAY 
FPLC SEPARATION 
ADA TLC NPT 
N2 
+ 
(’85%) 
N.D. 
+ (50%) 
N.D. 
N.D. 
AX25 
+ 
(50%) 
- 
- 
- 
- 
SAX 
+ 
(63%) 
- 
- 
- 
+/- 
CAX 
+ 
(82%) 
- 
- 
N.D. 
N.D. 
HAX 
+ 
(67%) 
- 
- 
- 
N.D. 
BAX 
N.D. 
- 
N.D. 
- 
N.D. 
TAX 
N.D. 
N.D. 
— 
N.D. 
Methods : 
DNA dot blot: DNA was prepared from individual foci, spotted 
onto nitrocellulose paper, and hybridized to vector-specific 
radiolabelled probe. 
ADA starch gel and Neomycin phosphotransferase (NPT) assay: 
Clarified cell lysates were separated electrophoretically , 
and the enzyme activity detected in an in situ colorimetric 
assay. 
FPLC separation: Clarified cell lysates were separated on a 
Mono Q ion-exchange column and peak ADA isoenzyme or NPT 
fractions were collected 
ADA TLC: The conversion of radiolabelled adenosine to inosine 
was determined following their separation by thin layer 
chromotography . 
[ 202 ] 
Recombinant DNA Research, Volume 12 
