C) Description of experimental animal procedures. 
Animals to be studies will be Rhesus monkeys currently located In the 
primate colony Buildings 14/3. 
PROTOCOL 
Day -6. The animal will be transferred to the P3 Primate Biohazard 
Facility. Building 36. 5C corridor. Studies In this facility will be under the 
supervision of Dr. William T. London. Chief Experimental Pathology Section, 
Infectious Diseases Branch. NINCDS. 
The animal will be acclimated to the facility for one week. During this 
time blood will be obtained from the animal on Wednesday and Friday (6 ml in a 
purple top tube for serum and 6 ml in a red top for plasma). Samples will be 
sent to the Laboratory of Molecular Hematology. Building 10. Room 7D-18 for 
evaluation under the direction of Dr. French Anderson. 
Day 0. Animal will be pretreated 30 minutes prior to virus Injection with 
2 mg/kg of Benadryl followed by 15 mg of hydrocortisone, each given IV over 5 
minutes. 10 ml/kg of the appropriate viral supernate will be given by IV 
Injection at 1 ml/min. Immediately. (1 minute) thereafter 12 ml of blood, (6 
ml In a red top and 6 ml In a purple top tube), will be obtained for 
evaluation. Demerol (1 mg/kg) will be ready for IV administration should the 
animal develop fever or rigors after supernate Injection. 
Every Monday. Wednesday and Friday that the animal Is within the Primate 
Biohazard Facility 12 ml of blood. (6 ml red/6 ml purple), will be obtained. 
When 2 consecutive S + /L~ assays (excluding day 0) are negative the animal 
will be returned to the primate colony. Buildings 14/3. under the supervision 
of Dr. Joe Pierce. 
After return to the colony, blood (6 6 ml) will be obtained 2 times per 
week for the first month, weekly for 6 months, then once every month thereafter. 
Recombinant DNA Research, Volume 12 [209] 
