bone marrow (and possibly liver) biopsies will be obtained every 6 months to 
monitor for viral production and the presence of the provirus. 
If after return to the colony the animal becomes vlremlc. It will be 
removed from the colony and (1) returned to the Primate Biohazard Facility In 
Building 36. or (2) placed In Isolation In Building 3 or (3) sacrificed. 
D) Sample Analysis 
Blood samples will be transported to Building 10. Room 7D-18 In a sealed 
plastic bag labeled biohazard. Studies will be performed under P2 conditions. 
Serum and plasma will be obtained after centrifugation of the blood 
sample. 1 ml of plasma will be processed for immediate assay by S + /L* (to 
detect the presence of replication competent retrovirus). 
The remaining serum and plasma will be frozen and stored In 1 ml aliquots 
for future use. 
S + /L" assays require 3-5 days. 
DNA analysis by Southern blot and/or PCR (for neo and ampho envelope 
fragment) will be performed to determine If the provirus can be detected and if 
recombination has occurred. 
E) Complications 
Potential complication Include: 
1) Injection of the viral supernate can potentially elicit a variety of 
allergic reactions. Including anaphylaxis or renal failure. The 
animal should be monitored at the time of Injection and thereafter 
for these complications. 
2) Amphotroplc retrovirus has the potential to Infect other primates. 
Proper protection methods should be taken by those who care for the 
animals and those who process the samples. 
3) If the animal becomes 111 It will be evaluated with the appropriate 
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Recombinant DNA Research, Volume 12 
