Genetics: KantofT et at. 
Proc. Nall. Acad. Sci. USA 83 11986) 6567 
that is a characteristic finding in SC1D. To our knowledge, 
the TJF-2 cell line represents the first ADA-deficient T-cell 
line available to assess ADA functional activity. It is not 
clear, however, what relation HTLV-I-immortalized T cells 
have to normal T cells, much less to the pluripotent stem cell 
(or the lymphoid-lineage stem cell) that would be the cell that 
needs to be corrected in viva in ADA deficiency. Nonethe- 
less. the experiments described here, together with the 
studies previously published (2-13). do provide encourage- 
ment that retroviral-based vectors may provide the clinical 
means for correcting ADA deficiency and other genetic 
diseases. 
We are indebted to Richard Hess. Sheri Bernstein. Jane Selegue. 
and Diana Hernandez for their technical assistance: to Rochelle 
Hirschhom and Steve O'Bnen for assistance with the starch gel 
electrophoresis: and to Cathy Magruder for assistance in the prep- 
aration of the manuscript. The TJF2 cells were derived from 
peripheral blood from James Fox. We wish to express our appreci- 
ation to his parents, who have shown continued support for this 
study. This work was presented in pan at the Workshop on Pnmary 
Immune Deficiency Diseases held in Cmunden. Austria. August 
19-21. 1985. E G. was funded in pan by a grant from the March of 
Dimes (1-8J6). 
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