hybridization of sequences in the bone marrow and peripheral blood, a light 
band was detected at 4.9 kb in the spleen lane (data not shown). 
FBMC obtained from animal #10 on day 69 were also analyzed for h-ADA and 
KPT activities. Fractionation of cell proteins by FPLC Mono Q ion exchange 
chromatography effectively separates human from priirate ADA as assessed by 
starch gel analysis of species specific ADA isozymes (21). In animal #10, 1% 
conversion of 1 4 C-labelled adenosine to 1 1 *C-inosine can be detected in the 
fractions in which human but not monkey ADA elutes. The human ADA activity 
detected was calculated to be less than 0.01% of the endogenous primate ADA 
activity (see legend Table 1) . NPT activity (which elutes from the column 
later, see Fig. 5) was also detected in the PBMC at lew levels at the same 
time. 
Supernatant Infection Protocol 
In the first two transplants there was poor recovery of bone marrow cells 
from the tissue culture dishes after the 24 hour co-cultivation period (Table 
1) . In an attempt to increase the number of cells recovered after transfer 
manipulations and to eliminate the possibility of S3A cell contamination, the 
subsequent two vector transfers (animals #56 and #57) were performed by 
incubating bone marrow cells with a filtered (0.22 v) supernatant collected 
from the SAX vector-producing S3A cells. Infection was for 2 hrs., a time that 
had been determined to be adequate to infect CFU-C in vitro (Figure 2) and to 
yield a good recovery of viable bone marrow progenitor cells (Table 1). The 
ratios of total infectious SAX virus (as measured by G4 18-res is tant cfu/ml) to 
total bone marrow cells were 8.3:1 and 4.9:1 in monkeys #56 and #57, 
respectively. The 14 day CFU-C analysis of an aliquot of the treated marrow 
cells shewed that 25% of the colonies were resistant to 1.7 mg/ml G418 for 
animal #56 (data not shown) and 28% for animal #57 (Figure 2, curve C). Both 
[286] Recombinant DNA Research, Volume 12 
