primates achieved a rapid and sustained return of normal hematopoietic function 
with full reconstitution of all blood cell lines entirely analogous to that 
observed following transplants of untreated autologous bone marrow cells. 
These animals survived and are healthy more than 1 year post transplantation. 
Hematopoiesis remains normal in these animals and no evidence of retroviremia 
has been detected by S+L” assay. 
Southern blot analyses (of DNA extracted from PRMC obtained on days 34 and 
52 as well as of ENA from bone marrow on day 104) for evidence of SAX vector 
sequences in these two animals were repeatedly negative. This suggests that 
the vector, if present, was inserted in less than 5% of cells, that is, below 
the limits of detection of the method used. PBMC were also analyzed for h-ADA 
activity and for NFT activity. Both monkeys demonstrated low, but readily 
detectable levels of h-ADA at several time points during the post-transplant 
course (Table 1). FBMC from animal #56 contained a level of h-ADA activity 
equivalent to 0.2% of the endogenous primate activity at day 104j in animal 
#57, the peak levels of h-AEA on day 69 brought about a 66% conversion of 
1 *C-labelled adenosine to 1 *C-inosine (Table 1) which was calculated to be 0.5% 
of endogenous primate ADA activity. The separation of human (fractions 2-4) 
frcm primate (fractions 16-25) ADA using FPLC and the levels of human and 
primate ADA activity detected in animal #57 at day 69, expressed as percent 
adenosine to inosine conversion, are presented in the top panel of Figure 5. 
These results are contrasted with those obtained frcm a control animal that 
received uninfected autologous bone marrow: this animal (as well as several 
other control aninals) had no detectable activity in the column fractions that 
would contain h-ADA (Figure 5, lower panel) despite greater total protein being 
loaded onto the column. Sequential assays of FBMC frcm animals #56 and #57 for 
h-ADA (Figure 6) demonstrated h-ADA activity between days 60 and 120 h-ADA was 
Recombinant DNA Research, Volume 12 [287] 
