Table 2 Resistant bone marrow colonies (CFU-C) assayed at the time of 
infection and post-transplantation 
PRIMATE CFU-C CFU-C CFU-C 
MARROW CELLS PRETRANSPLANT DAY 28 POST DAY 169 POST 
TRANSPLANT TRANSPLANT 
G418 
(mq/ml) CONCENTRATION 
0 
0.29 
1.71 
0 
0.29 
1.71 
0 
0.29 
1.71 
Uninfected 
control 
37 
0 
0 
26 
0 
0 
55 
0 
0 
Monkey #56 
56 
17 
14 
153 
5 
0 
61 
1 
0 
Monkey #57 
41 
12 
12 
ND 
ND 
ND 
53 
0.6 
0 
Bone marrow cells were harvested, processed and incubated with the supernatant 
frctn S3A producer cells that generate viral particles (containing the SAX 
retroviral vector) at a titer of 2 x 10^ cfu/ml, as described in the legends of 
figures 2 and 3. After infection, cells were iranediately plated in increasing 
concentrations of G418-containing CFU-C medium. For the post-transplant time 
points, bone mar rev mononuclear cells were collected and prepared as described 
and directly plated into the G41 8-con taining CFU-C medium. The values of 
colonies are reported for 1 x 10^ cells. The number of cells plated were 3 xr 
10 5 for the controls (no G418) and 1.2 x 10 6 for G418- containing points. H), 
not determined. 
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Recombinant DNA Research, Volume 12 
