FIGURE LEGENDS 
Figure 1 Map of SAX vector. The following regions are indicated: 0-1.5 and 
4. 7-5. 5 kb, Moloney murine leukemia virus sequences 1.5-2. 9 and 4. 6-4. 7 kb, 
neomycin -resistance gene (neo^) from Tn5 transposon (the hatched area is the 
coding sequence) 2. 9-3. 3 kb, Kpn I -Hind III fragment of the SV40 early 
promoter 3. 3-4. 6 kb, human ADA cDNA (h-ADA, black box) LTR, viral long 
terminal repeat 5', 5' donor splice site viral packaging signal. 
Restriction sites: S, Sac I K, Kpn I P, Pst I E, Eco RI C, Cla I. 
Figure 2 Susceptibility of cyncmolgus primate nyeloid progenitors (day 14 
CFU-C) to increasing concentrations of the neonycin analogue G418. Curve 
A: survival of control non-inf ected colonies. Curve B: survival after a 24 
hour cocultivation of bone marrow cells on a monolayer of SAX -producing NIH 
3T3 cells. Curve C: survival of marrow cells from animal #57 after a 2 
hour incubation with cell-free supernatants containing the SAX vector at a 
6 R 5 
titer of 2 x 10 neo cfu/ml. Triplicates of 1 x 10 bone marrow 
mononuclear cells/plate were utilized for each experimental point. The 
total number of colonies in the absence of G418 was 36-58/plate. The 
indicated concentrations of G418 (active j dry weight is twice as high) were 
added to the culture system immediately before plating. Colonies were 
counted on day 14. 
Recombinant DNA Research, Volume 12 
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