Figure 3 Hematopoietic reconstitution of primates post-transplant. 
Panel A : the hematopoietic reconstitution of white blood cells (open 
symbols) and platelets (closed symbols) of primates #10 (■ i Q) and #855 
( • > ° ) , whose marrow cells were cocultivated for 24 hours on a monolayer 
of SAX -producing NIH 3T3 cells. After coculture the nonadherent cells 
were washed with phosphate-buffered saline (PBS) and infused back into the 
animal. Panel B: the hematopoietic reconstitution of primates #56 
( m , O ) and #57 ( • , o ) who received autologous bone marrow cells 
infected during a 2 hour incubation with a cell-free supernatant 
containing SAX vector at a titer of 2 x 10 neo cfu/ml. Two additional 
primates described in this paper were also transplanted with a similar 
protocol (Table 1 - primates #77 and #78) ? their pattern of 
reconstitution was similar to those of primates #56 and #57. 
Figure 4 Southern blot of DNA extracted from tissues obtained at autopsy on 
day 69 f ran animal #10. DNA was prepared from spleen (S) , bone marrow 
(M) and blood (B) . Lane 1 (-) contains 30 g of DNA prepared from 
peripheral blood mononuclear cells of a control private. Lane 2 (+) 
contains 5 pg of the plasmid SAX mixed with 30 y g control private DNA 
digested with Kpn I. Lanes 3 (S), 4 (M), and 5 (B) contain 30 yg of ENA 
prepared from spleen, bone narrow and blood of animal #10. To determine if 
the 4.9 kb band in lanes 4 and 5 might be due to contaminating plasmid DNA, 
the DNA samples were re-assayed but without Kpn I digestion. The band of 
positive hybridization then appeared at the origin with high molecular 
weight DNA. 
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Recombinant DNA Research, Volume 12 
