Figure 5 FPLC fractionation of hematopoietic mononuclear cell lysates f ran 
animal #57 and a non-inf ected control animal . ADA activity (both 
endogenous monkey and vector -derived human) in primate #57 bone marrcw 
cells 69 days after transplantation is shown in the upper panel. An 
uninfected control is shown in the lever panel. The graph represents the 
absorbance at 280 nm of bone marrow cell lysate fractionated fcy ion 
exchange chromatography on an FFLC Mono Q column using a KC1 
salt gradient. Stippled bars represent ADA activity expressed as the 
percent conversion of l^-adenosine to ^^-inosine (%A - — ► I) . Following 
thin layer chromatographic separation of adenosine and inosine, the raw 
14 
data for fraction 3 (66% conversion) was 9045 cpm in the C-a denosine 
spot, and 17,889 cpra in the * ^-inosine spot. Background counts were: 407 
cpm for * 4 C-adenosine after 100% conversion (viz., fractions 17-18) and 
426 cpn for ^ 4 C-inosine after 0% conversion (viz., fraction 1). Fractions 
p 
containing human ADA, monkey ADA or neo activity are indicated. 
Figure 6 The h-ADA activities following FFLC fractionation detected in animals 
#56 and #57 over time after total body irradiation (TBI), retroviral gene 
transfer and bone marrow transplantation CBJfT) . The data point at day 69 
for animal #57 was taken from Figure 5 expressed as percent conversion of 
14 14 
C -adenosine to C-inosine. 
Figure 7 The neo phosphotransferase (NFT) activity detected in animals #56 
and #57 on day 52 and day 104. Bone marrow samples were taken on day 52 
and peripheral blood samples were obtained on day 104. The lanes denoted 
by (+) contained lysate of 5 x 10 5 3T3 cells that had been infected with 
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