the N2 vector. The d52 lames denoted by #56 aind #57 contained lysate of 1 
x 10 7 bone marrow cells from day 52. The dl04 lanes denoted as FB 
contained lysates of 1 x 10 7 peripheral blood cells and the lanes denoted 
as Col. were the neo^ fractions from an FPLC Mono Q column that had been 
used to fractionate 1 x 10 8 peripheral blood cells (see Fig. 5). The 
lanes denoted by. (-P) and by d52 #56 and #57 demonstrate the 29 kD band 
(the size of the NPT enzyme as determined by previous experiments). 
Figure 8 In situ hybridization of peripheral blood from animal #57 at day 127. 
Two positive cells (one, in a cluster of negative cells in the lower left 
hand portion of the figure, and the other at the upper edge on the right) 
showing overlying silver grains produced by hybridization of an 
35 p 
S-labelled-neo probe to vector-derived mRNA are shown. There were no 
positive cells found on slides made from uninfected control primate FBMC. 
Figure 9 Limiting dilution analysis of clonable G418-resistant T cells from 
peripheral blood mononuclear cells from monkey #56 collected on day 181 
after autologous transplantation of SAX infected marrow. The fraction of 
negative wells was determined by microscopic examination on day 10 of 
culture. The slope of line A is the frequency (1/7) of clonable T cells 
in the absence of G418. The slope of line B is the frequency (1/93) of 
clonable T cells in the presence of 0.1 mg /ml of G418. This concentration 
of G418 completely inhibited growth of T cells isolated from non-inf ected 
animals (i.e., no positive wells in 4 separate control experiments). 
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Recombinant DNA Research, Volume 12 
