Federal Regiater / Vol. 47. No. 167 / Friday. August 27. 1982 / Notices 
38057 
Appendix B. — Classification of 
Microorganisms on the Basis of Hazard 
Appendix B-1. Classification of 
Etiologic Agents. (The original reference 
for this classification was the 
publication "Classification of 
Etiological Agents on the Basis of 
Haza^.” 4th edition. July 1974, U.S. 
Department of Health. Education, and 
Welfare, Public Health Service, Center 
for Disease Control. Office of Biosafety, 
Atlanta, Georgia 30333. For the purposes 
of these Guidelines, this list has been 
revised by the NIH.J 
Appendix B-I-A. Class 1 Agents. All 
bacterial, parasitic, fungal, viral, 
rickettsial, and chlamydial agents not 
included in higher classes. 
Appendix tf-I-B. Class 2 Agents. 
Appendix B-l-B-1. Bacterial Agents. 
Acinetobacter calcoacelicua 
AcUnobacH/us — all species 
Aeromonaa hydrophila 
Arizona hinahawii — all serotypes 
BocHlua anthmcia 
Bordetella — all species 
Bomlia recumntia, B. vincenti 
Campylobacter fetua 
Campylobacter jejuni 
Chlamydia paittaci 
Chlamydia trachomatia 
Cloatridium botulinum. Cl. cltauvoei. Cl. 
haemolyticum. Cl. hialalyticum. Cl. novyi. 
Cl. aepticum. Cl. tetani 
Corynebaclerium diphtherias. C egui, C 
haemolytltum, C- paeudotubsrculoaia. C 
pyogenea. C renale 
Bdwardaiella tarda 
Eryaipelothrix inaidioaa 
BKherichia coli — all enteropalhogenic. 
enterotoxigenic, enleroinvasive and strains 
bearing Kl antigen 
Haemophilua ducreyi, H. inPuenzae 
Klebsiella — all species and all serotypes 
Legionella pneumophila 
Leptospira interrogans — all serotypes 
Uateria — all species 
Moraxella — all species 
Mycobacteria — all species except those 
listed in Class 3 
Mycoplaa.ma — all species except 
Mycoplaama mycoidea and Mycoplaama 
ogalacliae. which are in Class 5 
Neiaaerin yonorrboeoe. N. meningitidia 
Paateurr'ht — all species except those listed in 
Class 3 
Salmonella — all species and all serotypes 
Shigella — all species and all serotypes 
Sphaerophorua necrophorua 
Slaphylococcua aureus 
Streptobocillua moniliformis 
Streptococcus pneumoniae 
Streptococcus pyogenes 
Treponema carateum. T pallidum, and T. 
pertenue 
Vibrio cholerae 
Vibrio parahemolyticus 
Yersinia enterocolitica 
Appendix B-l-B-2. Fungal Agents. 
Actinamycetes (including Nocardia species 
and Actinomyces species and Arachnia 
propionica) 
Blastomyces dermatitidis 
Cryptococcus neoformans 
Paracoccidioides braziliensis 
Appendix B-I-B-3. Parasitic Agents. 
Endamoeba histolytica 
Leishmania sp. 
Naegleria gruberi 
Schistosoma manaoni 
Toxoplasma gondii 
Toxocaro cam's 
Trichinella spiralis 
Trypanosoma cruzi 
Appendix B-I-B-4. Viral. Rickettsial, 
and Chlamydial Agents. 
Adenoviruses — human — all types 
Cache Valley virus 
Coxsackie A and B viruses 
Cytomegaloviruses 
Echovirusea — all types 
Encephalomyocarditia virus (EMCj 
Flanders virus 
Hart Park virus 
Hepotitis-sssocisied antigen material 
Herpes viruses— except Herpesvirus aimioe 
(Monkey B virus} which is in Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8/ 
34. which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3. SF4 strain, which is 
in Class 1 
Polioviruses — all types, wild and attenuated 
Poxviruses — all types except Alostrim, 
Small-pox, and Whitepox, which are Class 
S and Monkey pox. which depending on 
experiments, is in Class 3 or Class 4 
Rabies virus — all strains except Rabies street 
virus, which should be classified in Gass 3 
Reoviruaes — all types 
Respiratory syncytial virus 
Rhinovirusea — aU types 
Rubella virus 
Simian viruses — all types except Herpesvirus 
aimioe (Monkey B virus} and Marburg 
virus, which are in Class 4 
Sindbis virus 
Tensatv virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vesicular stomatitis virus |3) 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
Appendix B-l-C Class 3 Agents. 
Appendix B-l-C-1. Bacterial Agents. 
Bartonella — all species 
Brucella — all species 
Francisello tularensis 
Mycobacterium avium. M. bovis. M. 
tuberculosis 
Poateurella multocide type B ("buffalo’' and 
other foreign virulent strains) |3| 
Pseudomonas mallei (3) 
Pseudomonas pseudomallei [3] 
Yersinia pestis 
Appendix B-l-C-2. Fungal Agents. 
Coccidioidea immitis 
Hiatoplasma copsulatum 
Histoplasma copsulatum var. duboisii 
Appendix B-I-C-3. Parasitic Agents. 
None. 
Appendix B-l-C-4. Viral, Rickettsial, 
and Chlamydial Agents. 
Monkey pox. when used in \itro 14| 
Arboviruses — all strains except those in 
Class 2 and 4 [Arboviruses indigenous to 
the United States are in Class 3. except 
those listed in Class 2. 
West Nile and Semliki Forest viruses may be 
classiTied up or down, depending on the 
conditions of use and geographical location 
of the laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Rabies street virus 
Rickettsia — all species except Vole rickettsia 
when used for transmission or animal 
inoculation experiments 
Yellow fever virus — wild, when used in vitro 
Appendix B-1— D. Class 4 Agents. 
Appendix B-l-D-1. Bacterial Agents. 
None 
Appendix B-I-D-2. Fungal Agents. 
None 
Appendix B-I-D-3. Parasitic Agents. 
None 
Appendix B-I-D-4. Viral, Rickettsial, 
and Chlamydial Agents. 
Ebola fever virus 
Monkey pox. when used for transmission or 
animal inoculation experiments (4) 
Hemorrhagic fever agents, including 
Crimean, hemorrhagic fever, (Congo). 
Junin, and Machupo viruses, and others as 
yet undefined 
Herpesvirus aimiae (Monkey B virus) 
Laaaa virus 
Marburg virus 
Tick-borne encephalitis virus complex, 
including Russian spring-summer 
encephalitis, Kyosonur, forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
Venezuelan equine encephalitis virus. 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal inoculation 
experiments 
Appendix B-II. Classification of 
Oncogenic Viruses on the Basis of 
Potential Hazard fSj. 
Appendix B-Il-A. Low-Risk 
Oncogenic Viruses. 
Rous Sarcoma 
SV-40 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tumor 
Avian Leukosis 
Murine Leukemia 
Murine Sarcoma 
Mouse mammary tumor 
Rat Leukemia 
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