38062 
Federal Register / Vol. 47, No. 167 / Friday, August 27, 1982 / Notices 
Appendix G-U-B-l-k. An insect and 
rodent control program shall be 
instituted. 
Appendix G-Q-B-1^. The use of 
laboratory gowns, coats, or uniforms is 
required. Laboratory clothing shall not 
be worn to the lunch room or outside of 
the building in which the laboratory is 
located. 
Appendix G-lI-B-l-m. Animals not 
related to the experiment shall not be 
permitted in the laboratory. 
Appendix G-11-B-l-n. Use of the 
hypodermic needle and syringe shall be 
avoided when alternative methods are 
available. 
Appendix G-II-B-l-o. The laboratory 
shall be kept neat and clean. 
Appendix G-Il-B-l-p. Experiments of 
lesser biohazard potential can be 
carried out concurrently in carefully 
demarcated areas of the same 
laboratory. 
Appendix G-U-B-2. Containment 
Equipment. Biological safety cabinets 
[12] shall be used to contain aerosol- 
producing equipment, such as blenders, 
lyophilizers, sonicators, and centrifuges, 
when used to process organisms 
containing recombinant DNA molecules, 
except where equipment design 
provides for containment of the 
potential aerosol. For e^cample; a 
centrifuge may be operated in the open 
if a sealed head or safety centrifuge 
cups are used. 
Appendix G-II-B-3. Special 
Laboratory Design. An autoclave for 
sterilization of wastes and contaminated 
materials shall be available in the same 
building in which organisms containing 
recombinant DNA molecules are used. 
Appendix G-II-C. P3 Level. 
Appendix G-U-Ol. Laboratory 
Practices. 
Appendix G-II-C-l-a. Laboratory 
doors shall be kept closed while 
experiments are in progress. 
Appendix G-Il-C-l-b. Work surfaces 
shall be decontaminated following the 
completion of the experimental activity, 
and immediately following spills of 
organisms containing recombinant DNA 
molecules. 
Appendix G-II-C-l-c. All laboratory 
wastes shall be steam-sterilized 
(autoclaved) before disposal. Other 
contaminate materials, such as 
glassware, animal cages, laboratory 
equipment, and radioactive wastes, 
shall be decontaminated by a method 
demonstrated to be effective before 
washing, reuse, or disposal. 
Appendix G-H-C-l-d. Mechanical 
pipetting devices shall be used; pipetting 
by mouth is prohibited. 
Appendix G-II-C-l-e. Eating, 
drinking, smoking, and storage of food 
are not permitted in the laboratory area 
in which recombiant DNA materials are 
handled. 
Appendix G-II-C-l-f. Persons shall 
wash their hands after handling 
organisms containing recombinant DNA 
molecules and when they leave the 
laboratory. 
Appendix G-U-C-l-g. Care shall be 
exercised to mi nimiz e Oie creation of 
aerosols. For example, manipulations 
such as inserting a hot inoculating loop 
or needle into a culture, flaming an 
inoculation loop or needle so that it 
splatters, and forceful ejection of fluids 
from pipettes or syringes shall be 
avoided. 
Appendix G-II-C-1^. Contaminated 
materials that are to be steani-sterilized 
(autoclaved) or decontaminated at a site 
away from ^e laboratory shall be place 
in a durable leak-proof container, which 
is closed before removal from the 
laboratory. 
Appendix G-H-C-l-i. Entry into the 
laboratory shall be through a controlled 
access area. Only persons who have 
been advised of the nature of the 
research being conducted shaU enter the 
controlled access area. Only persons 
required on the basis of program or 
support needs shall be authorized to 
enter the laboratory. Such persons shall 
be advised of the nature of the researdi 
being conducted before entry, and shall 
comply with all required entry and exit 
procedures. 
Appendix G-U-C-l-j. Persons under 
16 years of age shall not enter the 
laboratory. 
Appendix G-II-C-l-k. The universal 
biohazard sign shall be posted on the 
controlled access area door and on all 
laboratory doors when experiments 
requiring P3-level containment are in 
progress. Freezers and refrigerators or 
other units used to store organisms 
containing recombinant DNA molecules 
shall also be posted with the universal 
biohazard sign. 
Appendix G-D-C-l-l. An insect and 
rodent control program shall be 
instituted. 
Appendix G-II-C-l-m. Laboratory 
clothing that protects street clothing 
(e.g., long-sleeve solid-front or wrap- 
around gowns, no-button or slipover 
jackets) shall be worn in the laboratory. 
Front-button laboratory coats are 
unsuitable. Laboratory clothing shall not 
be worn outside the laboratory and shall 
be decontaminated before it is sent to 
the laundry. 
Appendix G-II-C-l-n. Raincoats, 
overcoats, topcoats, coats, hats, caps, 
and such street outer-wear shall not be 
kept in the laboratory. 
Appendix G-II-C-l-o. Gloves shall be 
worn when handling materials requiring 
P3 containment. They shall be removed 
aseptically immediately after the 
handling procedure and 
decontaminated. 
Appendix G-II-C-l-p. Animals and 
plants not related to the experiment 
shall not be permitted in the laboratory. 
Appendix G-II-C-l-q. Vacuum 
outlets shall be protected by Biter and 
liquid disinfectant traps. 
Appendix G-II-C-l-r. Use of 
hypodermic needle and syringe shall.be 
avoided when alternative methods (Sre 
available. 
Appehdix G-II-C-l-s. The laboratory 
shall be kept neat and clean. 
Appendix G-II-C-l-t. If experiments 
involving other organisms which require 
lower levels of containment are to be 
conducted in the s£ime laboratory 
concurrently with experiments requiring 
P3-level physical containment, they 
shall be conducted in accordance with 
all P3-level laboratory practices. 
Appendix G-II-C-2. Containment 
Equipment. 
Appendix G-Il-C-2-a. Biological 
safety cabinets [12] shall be used for all 
equipment and manipulations that 
produce aerosols — e.g., pipetting, 
dilutions, transfer operations, plating, 
flaming, grinding, blending, drying, 
sonicating, shaking, centrifuging — ^where 
these procedures involve organisms 
containing recombinant DNA molecules, 
except where equipment design 
provides for containment of the 
potential aerosol. 
Appendix G-II-C-2-b. Laboratory 
animals held in a P3 area shell be 
housed in partial-containment caging 
systems, such as Horsfall units [11], 
open cages placed in ventilated 
enclosures, solid-wall and -bottom cages 
covered by filter bonnets, or solid-wall 
and -bottom cages placed on holding 
racks equipped with ultraviolet 
radiation lamps and reflectors. (Note: 
Conventional caging systems may be 
used, provided that all personnel wear 
appropriate personal protective devices, 
liiese shall include, at a minimum, 
wrap-around gowns, head covers, 
gloves, shoe covers, and respirators. All 
personnel shall shower on exit from 
areas where these devices are required.) 
Appendix G-II-C-2-c. Alternative 
Selection of Containment Equipment. 
Experimental procedures involving a 
host-vector system that provides a one- 
step higher level of biological 
containment than that specified can be 
conducted in the P3 laboratory using 
containment equipment speciffed for the 
P2 level of physical containment. 
Experimental procedures involving a 
host-vector system that provides a one- 
step lower level of biological 
containment than that speciBed can be 
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