16 
between S. faecalis and sanguis . Dr. Cartpbell supported that approach, 
and ncve3~aK)roval. Dr. Fedoroff seconded the motiai. 
By a vote of twelve in favor, two c^xised, and five abstentions, the RAC 
recoraraended that the request be approved, and that Streptoooccus faecalis 
be added to sublist F of Af^ndix A. 
XII. PROPOSAL FOR FOR STREPTCMYCES AND NCNPAIHOGENIC ACTINOMYCErES 
Dr. Levine introduced three requests (tab 974) of Dr. Stanley Cohen of 
Stanford Uiiversi^ to revise oontainnent levels for recombinant DNA 
experiments involving the ncxpathogenic free-living soil organism genus 
Streptomyces and other nonpathogenic Actinomycetes . Dr. Talbot pointed 
out that this item had not been published in the Federal Register for 
thirty days of public camment due to its late receipt in ORDA. He noted 
however that the first of the three requests was a specific instance of 
a much broader proposal (see item VII of these minutes) which had been 
published in the Federal Register , and that, therefore, the RAC could appro- 
priately act CXI this. Dr. Levine read the first of Dr. Cchen's requests: 
"That all members of the ncxpathogenic Ac^incxtycetes genus 
Streptcmyc»s and the plasmids native to this ^nus be ^proved 
as host-vec±or systens for the clcxiing under PI conditions of ENA 
derived from other non-pathogenic prdlcaryotic organisms suoh as 
Streptonyoes and other ncx>-pathogenio Actinomycetes species, 
Eschericnia ooli K-12, Bacillus subtilis . Bacillus licdienformis . 
Bacillus circulans , and other n<x>-pathogenic Bacillus species, 
and for the clcxiing of DNA c3erived frxxn non-pathogen i c unicellular 
eu3caryotic microorganisms such as Sacchanxiyces cerevisiae and 
Neurospora cnrassa." 
Dr. Levine noted that the RAC had earlier in the meeting (see item VII 
of these minutes) recenmended PI containment as sufficient for a large 
class of experiments, of which these were a snail subset. He recommended 
^proval. Et. Maas secx>nded the motion. 
By a vote caf eighteen in favor, ncxae cDpposed, and two abstentions, the 
RAC recommended approval of the first request of tab 974. Dr. Campbell 
abstained from discussing and voting on this request. 
The RAC cSeferred action on the second and third requests of tab 974. 
Dr. Gottesnan said that Dr. Cohen should supply additional cfeta concerning 
the organisms he wishes to be certified as HVl host-vector systems. 
Dr. Williams cxxicurred. 
[ 44 ] 
