15 
of practice; (b) ocxitinued applicability to recombinant ENA 
technology; (c) currently recommended levels of ccxitainment; 
(d) current processes and procedures inpeding or facilitating 
research and/or industrial application. Such study group to 
report back to the RAC its findings, conclusions, and recom- 
mendations for RAC review and consideration. Solicitation of 
public input and ccninent beya>d publication in the Federal 
Register will be obtained." 
By a vote of fifteen in favor, four opposed, and two abstentions, the com- 
mittee accepted this substitute motion. Mr. Tliomton then called the vote 
on the motiOTi. Ey a vote of nineteen in favor, two opposed, the RAC adopted 
the motion. 
VII. PROPOSED OOWTAINMEMT CONDITIONS FOR FLIES 
Dr. McGarrity introduced the proposal (tab 1004) from Dr. 'nicroas Maniatis 
of Harvard University to transform Drosophila with the Drosoghila alcohol 
dehydrogenase gene. line clcxned DNA (in lambda, plasmid or cosmid vectors) 
will be injected into either the abdomen of adult female alcohol dehydro- 
genase deficient flies, or into early stage alcohol dehydrogenase deficient 
embryos, Ihe Guidelines allow such work to proceed at PI containment. Une 
Harvard IBC believes the safety measures proposed meet the requironents of 
PI, but asked for RAC concurrence. Une containment conditions include: 
(1) Bottles containing flies will be disposed of by autoclaving. (2) Flies 
will be propagated in bottles or vials, (3) Only experienoed personnel 
will handle the Drosoghila . (4) All manipulations of adult flies will ^be 
performed in a cold room. Tine cold environment will act as an anesthetic 
for the flies. (5) All propagation of transformed flies will be carried 
out in an approved PI laboratory. 
Dr. McGarrity said the precautions to be enplpyed appear to be adequate 
and reconmended aEpaxnval. Dr, McKinney said that installation of an air 
curtain on the entry to the oold room would provide an additional banrier 
against escape. Dr. Gottesraan felt the precautions specified in the pro- 
posal were adequate and moved acceptance of the proposal. Dr. Fedoroff 
seconded. By a vote of nineteen in favor, none exposed, and no abstentions, 
the RAC recommended adoption of the proposal. 
VIII. OONTAINMENr LEVELS FOR RECOMBINANT DNA EXPERIMENTS INVOLVING BACILLUS 
SUBTILIS 
Dr. Williams initiated review of the request (tabs 1005, 1007, 1011, 1015/VI, 
1016) submitted by Dr. Donald Dean of Onio State University. Dr. Dean 
requested consideration of the current classification of Bacillus subtilis 
host-vector systems. Dr. Dean's request ccaisisted of three parts: 
(1) that any asporogenous Bacillus subtilis strain which does^not 
revert to a sporeformer with a frequency greater than 10"' can 
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