17 
motion is limited to those vectors listed by Dr. Dean. Dr. Williams replied 
that it Mould not be limited. Dr. Talbot said that current certification 
specifications for ^ subtil is HVl systems require that data demonstrating 
a reversion frequency to aporogeny of less than 10""^, be evaluated by NIH; 
he questioned whether this specification was iiiplied under Dr. Williams' 
moticxi. Er. Williams said it was not. 
Dr. Qottesman asked if in permitting use of all indigenous vectors, RAC 
might inadvertently authorize the use of an extremely broad host range 
vector which might infect Bacillus pathogens. 
Dr. Dean said that his proposal oould be modified to eliminate the use of 
E^age vectors that infect CDC Class 2 Bacilli such as Bacillus anthracis . 
It was pointed out that Bacillus cereus also is a pathogen. Dr. Levine 
said that Bacillus cereus enterotoxin causes disease, particularly in 
Southeast Asia, where it is a major cause of enteric problems. Ihe organism 
produces and excretes a potent enterotoxin v^ich ccxitaminates foodstuffs. 
Dr. Qottesman suggested the phrase "indigenous plasmid and phage vectors, 
yihose host-range does not include Bacillus cereus and Bacillus anthracis" 
be substituted for the words "vectors indigenous bo B. subtills ." 
Dr. Williams accepted this modificaticxi of the motion. 
Mr. Thornton called for the vote on the amended motion. By a vote of 
twelve in favor, none opposed, and five abstentions, the RAC reccmmended 
the motion. 
IX. CERTIFICATE OF APPRECIATION TO MRS. BETTY BDTLER 
Mr. Thomtcxi announced that Mrs. Betty Butler, vho has worked for ORDA 
for many years, had recently accepted another position at NIH. Mr. Thornton, 
calling the attention of the ccnmittee to Mrs. Butler's many years of ser- 
vice to the RAC, vhich have contributed so much to its efficient functioning, 
presented to her a plaque in e^preciation of her services, signed by NIH 
Director Donald S. Fredrickson. 
X. EXPRESSION OF FOOT AND MOUTH DISEASE VIRUS PROTEINS IN SACCHAROMYCES 
CEREVISIAE, BACILLUS SUBTILIS, AND MAMMALIAN TISSUE CULTURE 
Dr. Bems said he preferred to divide the Qenentech, Inc., proposal (tabs 
999, 1015/VII) into four parts, each to be discussed separately. 
Dr. Bems first discussed the question of removirq additional clones of 
the E\oot and Mouth Disease Virus (FMEV) genome from the Plum Island Animal 
Disease Center. Hrevious NIH ^>proval of the project permitting clones 
obtained in ^ ooli K-12 to be removed from Plum Island, stipulated that 
the plasmids removed from Plum Island should not separately or collectively 
represent more than 75% of the FMDV genome, liifortunately, the plasmids 
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