18 
transferred frcn Plun Island to the Qenentech, Inc./ facilities in Qdi- 
fomia appeurently did not cxxitain the VP3 ocxJing region/ the VP3 protein 
being the predoroinant antigenic moiety for the virus. 
The VP3 region of severed FMCV aerologiced types were subsequently cloned 
on Plun Island. Genentech/ Inc./ now requests permission to remove these 
additional clones to their facilities in South San Francisco. If these 
clones are removed/ however/ more them 75% of the FMEV genome will have 
been shipped from Plun Island. Discussion between the RAC wor)ung group 
on FT1CV/ U5DA/ and Qenentech/ Inc./ led to the proposed that plasmids 
representing sequences to the right of beme pair 6000 be returned to Plun 
Island/ after which it would be permissible to ship fron Plun Island to 
Qenentech the pleianids of interest. 
It was pointed out that the RAC reconnendation at the last meeting/ accepted 
by the NIH Director/ edlows the working group to approve the removed of 
these clones from Plun Island without obtaining full RAC concurrence. 
Nevertheless/ Dr. Bems moved that Qenentech/ Inc./ be granted permission 
to return those clones representing the extreme right portion of the genome 
to Plun Island/ and in exchange be permitted to remove the requested clones 
containing the center of the genome. Er. MoGarrity seconded the motion. 
The motion was adopted by a vote of sixteen in favor/ none opposed/ and two 
abstentions. 
Dr. Bems proceeded to that portion of the request dealing with use of 
host-vector systems other than ^ ooli K-12. Dr. Bems said Qenentech/ 
Inc./ had requested permission to clone the FKJJ genome in ^ subtil is 
host-vector systens. A discussion between the RAC working group on PMU/ 
and representatives of Qenentech/ Inc./ led to agreement that this would 
be limited to those portions of the FMIV genome lying between base pairs 
500 and 4/100. He suggested that PI conditions are adequate and so moved. 
Dr. McQarrity seconded the motion. By a vote of sixteen in favor/ none 
opposed/ and two abstentions/ the motion was adopted. 
Dr. Bems said the sane type of experiment was proposed utilizing Sacchar- 
omyoes oerevisiae host-vector systems. He again suggested that the experi- 
ments be permitted under Pi physical oontainment conditions if the subgencmic 
FMEV segments were restricted to those sequences «#iich map between 500 and 
4/100 of the FMEV genome. Dr. McQarrity seconded the motion. By a vote 
of sixteen in favor, none opposed, and two abstentions, the RAC adopted 
the proposed. 
Dr. Bems then turned the discussion to the proposal to clone portions of 
the FMEV genome, using the SV40 genome as a vector, in mammalian cell 
culture. Et. Bems noted that tissue culture systems are suitable "hosts" 
for large nurbers of different types of pioomavi ruses. He questioned 
whether an adventitious recombination between a contaminating picomavirus 
and the hybrid SV40 - FMEV molecule might occur. Dr. Baltimore -said evi- 
dence demonstrating reocmbination among homologous picomaviruses is 
marginal. He did not know of e:q»riments in the literature looking for 
1107] 
