20 
XII. (XNTAD#1ENr LEVELS FOR RBOOMBINANr ENA EXPERIMENTS INVOLVING NEURDSPORA 
CRASSA 
Dr. Brill introduced the request (tabs 995, 1015/III) from Dr. David Perkins 
of Stanford Dhiversity. Dr. I^rkins proposed that the following language 
be substituted for entry 2 of Appendix E: 
"Unnodified laboratory strains of Neurospora crassa can be used in 
adl experijnents for which HVl ^ crassa systems are approved, provided 
that only ENA from Class 1 agents is used. For agents other than 
Class 1, unmodified laboratory strains of crassa can be used in all 
experiments for which HVl ^ crassa systems are approved, providing 
that these are ceurried out at physical containment one level higher 
than required for HVl. However, if P3 containment is specified for 
HVl crassa , this level is considered adequate for unmodified N. 
crassa . Care must be exercised to prevent aeri^Ll dispersion of 
macroconidia in accordance with good laboratory practice. 
•Mutationally modified strains of N. crassa specified as HVl in Appendix 
D can be used in all experiments Tor which HV2 ^ crassa systems are 
approved, provided that only ENA from Class 1 agents is used." 
[)r. Brill said that N. crassa is not known to be a pathogen. It is not 
closely eissociated with nan or other organisms in nature. It produces no 
known toxins. He moved acceptance of the request. By a vote of ten in 
favor, none opposed, and five abstentions, the RAC adopted the motion. 
XIII. REQUEgr TO EMPLOY A OCKJUGXTIVE PtASMID TO TRANSFER NEUROSPORA CRASSA ENA 
Dr. Qottesman introduced the request (tabs 1(X)6, 1015/XIII) of ft:. Norman 
Giles of the Uhiversity of Georgia. Er. Giles requested permission to use 
a conjugative plasmid to transfer the Neurospora crassa qa-2 gene among 
E. ooli K-12 strains. The qa-2 gene would be ligated into a derivative 
oF the mobilizable plasmid ^F2124. The work would be performed under P2 
containment conditions. 
Dr. Qotteaian said that ed though this request involved the use of a conju- 
gative pl^lsmid, she would support the proposal as the crassa qa-2 gene 
is a relatively well-defined ETA fragment. 
Dr. Qottesman moved approval of the proposed. By a vote of seventeen 
in favor, none opposed, and no abstentions, RAC adopted the motion. 
XIV. RECXJEgr TO USE AN E. OOLI gTRAIN CONTAINING A Mu PHAGE INSERTION 
Dr. Goldstein introduced the request (tabs 1009, 1015AH) of Dr. Darold 
Holten of the Uhiversity of California at Riverside. Dr. Holten requested 
permission to utilize the ^ ooli K-10 strain DF214 (or derivatives thereof), 
and EK plasmid vectors (e.g. ,~p®322, pBR325) to clone rat cENA. Strain 
(1091 
